Proteins in binary solvents

Biophysics Reviews
Francesco SpinozziMaria Grazia Ortore

Abstract

Proteins in living organisms exist in complex aqueous solutions or embedded in membranes. In solution, proteins are surrounded by a tightly bound hydration layer, which is more ordered and less mobile than bulk water. As a consequence, water plays a major role in controlling protein structure stability, conformational flexibility, dynamics, and functionality, but it also appears that protein surface regulates the structuring of the surrounding water. The presence of cosolvents can modify the hydration layer characteristics and then the whole protein structural and dynamical properties. Because cytoplasm or biological liquids are complex solutions, the knowledge of the solvation shell characteristics in mixed solvents should be considered as a crucial step in describing biological processes at molecular level. This review reports on recent studies on the structural and thermodynamic properties of model proteins dissolved in binary solvent mixtures by small-angle neutron scattering (SANS) and differential scanning microcalorimetry (DSC) techniques. We will show that contrast variation SANS experiments allow to acquire a direct knowledge of both protein structure and protein solvation shell (in terms of low-resolution shape and so...Continue Reading

References

Jan 25, 1974·Journal of Molecular Biology·R Diamond
May 23, 1972·Biochemistry·C Tanford, R Roxby
Sep 24, 1982·Science·P H YanceyG N Somero
Aug 1, 1994·Biopolymers·J A Schellman
Feb 28, 1997·Biophysical Chemistry·G Xie, S N Timasheff
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·R V Rariy, A M Klibanov
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·D I SvergunG Zaccai
Feb 24, 2001·Nature·A M Klibanov
Jul 18, 2001·Journal of the American Chemical Society·M IkeguchiK Shimizu
Jan 25, 2002·Acta Crystallographica. Section D, Biological Crystallography·Franci Merzel, Jeremy C Smith
Feb 14, 2002·Journal of the American Chemical Society·Qin ZouKenneth P Murphy
Mar 28, 2002·Biophysical Journal·Francesco SpinozziFlavio Carsughi
Jul 19, 2002·Biophysical Journal·Alessandro PaciaroniGiuseppe Onori
Sep 23, 2003·Biophysical Chemistry·Serge N Timasheff, Guifu Xie
Sep 23, 2003·Biophysical Chemistry·A K SoperAlenka Luzar
Nov 25, 2003·Nature Structural Biology·Helen BermanHaruki Nakamura
Feb 18, 2005·Faraday Discussions·Akihiro Wakisaka, Takahiro Ohki
Jun 28, 2005·Biophysical Journal·Marc Niebuhr, Michel H J Koch
Feb 24, 2006·The Journal of Physical Chemistry. B·Alan K SoperJohn L Finney
Mar 4, 2006·The Journal of Chemical Physics·Matteo BroccioSow-Hsin Chen
Mar 11, 2006·Quarterly Reviews of Biophysics·John A Schellman
Oct 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·J Juraszek, P G Bolhuis
Mar 1, 2007·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Nadeem JavidRoland Winter
Jun 30, 2007·The Journal of Chemical Physics·Raffaele SinibaldiPaolo Mariani
Mar 28, 2008·European Biophysics Journal : EBJ·Raffaele SinibaldiPaolo Mariani
Sep 25, 2008·The Journal of Physical Chemistry. B·Maria Grazia OrtorePaolo Mariani
Apr 23, 2009·Journal of Molecular Biology·Cristiano Luis Pinto OliveiraJan Skov Pedersen
Jul 3, 2009·Journal of the Royal Society, Interface·Maria Grazia OrtoreDaniela Russo
Sep 8, 2010·Biophysical Journal·Jouko J VirtanenKarl F Freed
Dec 1, 2010·The Journal of Physical Chemistry. B·Yun LiuPiero Baglioni
Sep 7, 2011·Physical Chemistry Chemical Physics : PCCP·Giuseppe Graziano
Oct 25, 2011·The Journal of Physical Chemistry. B·Francesco MallamaceSow-Hsin Chen
Nov 17, 2011·International Journal of Biological Macromolecules·Giuseppe Graziano
Nov 25, 2011·Angewandte Chemie·Martin A SchroerRoland Winter
Jan 10, 2012·The Journal of Chemical Physics·Maria Grazia OrtoreFrancesco Spinozzi
Jun 1, 2001·Journal of Biological Physics·S M VaianaM U Palma

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