PMID: 1525Nov 1, 1975

Proteins in the luminal fluid from the bovine oviduct

Journal of Reproduction and Fertility
G P RobertsH W Symonds

Abstract

Oviducal fluid was collected by cannulation from four cows and by irrigation from fifteen slaughtered cows. The proteins in the fluid were examined by polyacrylamide gel electrophoresis at pH 4-5 and pH 8-9, isoelectric focusing on polyacrylamide, immunodiffusion, immunoelectrophoresis, affinity chromatography and gel filtration. The macromolecular components found were mainly serum proteins but small amounts of other proteins were detected in oestrous and dioestrous samples by electrophoresis at pH 8-9 following fractionation of the fluid by gel filtration or affinity chromatography. Small amounts of cathodically migrating proteins were detected directly by electrophoresis at pH 4-5 in dioestrous samples but not in oestrous samples. Determination of glycosidase activities revealed that the levels at oestrus were similar to the levels detected in serum. At dioestrus, the activities of B-N-acetylgalactosaminidase and beta-N-acetylglucosaminidase were elevated.

Citations

Jan 1, 1977·European Journal of Obstetrics, Gynecology, and Reproductive Biology·R H Huntar
Mar 1, 1983·Prostaglandins·H Rodriguez-MartinezH Kindahl
May 1, 1979·Theriogenology·L G Paisley, W Duane Mickelsen
Aug 30, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Luis Cesar CarrascoPilar Coy
Aug 29, 2012·Animal Reproduction Science·Dessie Salilew-WondimDawit Tesfaye
Aug 18, 2009·Theriogenology·M A VelazquezH Niemann
Aug 1, 2012·Reproduction in Domestic Animals = Zuchthygiene·U BesenfelderG Brem
Dec 17, 2014·The Veterinary Journal·Jon Romero-AguirregomezcortaPilar Coy
Oct 1, 1994·Molecular Reproduction and Development·R H Hunter
Sep 1, 1995·Microscopy Research and Technique·F Gandolfi
Nov 1, 1995·The Anatomical Record·I Walter
Oct 11, 2007·Molecular Reproduction and Development·S A HugentoblerD G Morris
Oct 1, 1990·The Journal of Experimental Zoology·R L Gerena, G J Killian
Oct 10, 2008·Reproduction, Fertility, and Development·Luis Cesar CarrascoRaquel Romar
Jan 8, 2020·International Journal of Molecular Sciences·Marie Saint-DizierPascal Mermillod
Jan 1, 1988·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·J F Kavanaugh, G J Killian

Related Concepts

Beta-N-Acetylglucosaminidase
Serum Proteins
Body Fluids
Bos indicus
Chromatography, Affinity
Estrus
Fallopian Tubes
Exoglycosidases
Hexosamines
Hydrogen-Ion Concentration

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