PMID: 69719Jul 1, 1977Paper

Proteins of Rous-associated virus type 61: polypeptide stoichiometry and evidence that glycoprotein gp35 is not a cleavage product of gp85

Journal of Virology
A G MosserR R Rueckert

Abstract

The two glycoproteins, gp85 and gp35, of Rous-associated virus type 61 (RAV-61), were isolated from radiolabeled virions by gel electrophoresis and digested with trypsin. The chromatographic profile of the gp35 digest revealed no peaks in common with that of gp85; therefore, the smaller glycoprotein is not a cleavage product of gp85. The stoichiometry of radiolabeled RAV-61 proteins was studied by quantitative gel filtration and gel electrophoresis. Among the 11 polypeptides identified were 4 minor ones, including the beta(p91) and alpha(p64) chains of reverse transcriptase and two unidentified chains, p76 and p35; the latter two were unmasked by removing the virions' surface glycoproteins with a protease, bromelain. Virions contained some 15 to 30 molecules of reverse transcriptase.

References

Jan 1, 1975·Archives of Biochemistry and Biophysics·W H Porter, R J Winzler
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Citations

Oct 1, 1979·Molecular & General Genetics : MGG·R R BurgessP A Lowe
Jan 1, 1977·Medical Microbiology and Immunology·D P BolognesiS J Sullivan
Oct 1, 1979·Journal of Virology·C W RettenmierH Hanafusa
Aug 1, 1982·Journal of Virology·P J EnriettoR L Erikson
Jan 13, 1978·Science·D P BolognesiW Schäfer

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