PMID: 16615861Apr 18, 2006Paper

Proteins of the Rpf (resuscitation promoting factor) family are peptidoglycan hydrolases

Biochemistry. Biokhimii︠a︡
Miroslav TelkovArseny S Kaprelyants

Abstract

The secreted Micrococcus luteus protein, Rpf, is required for successful resuscitation of dormant "non-culturable" M. luteus cells and for growth stimulation in poor media. The biochemical mechanism of Rpf action remained unknown. Theoretical predictions of Rpf domain architecture and organization, together with a recent NMR analysis of the protein structure, indicate that the conserved Rpf domain has a lysozyme-like fold. In the present study, we found that both the secreted native protein and the recombinant protein lyse crude preparations of M. luteus cell walls. They also hydrolyze 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside, a synthetic substrate for peptidoglycan muramidases, with optimum activity at pH 6. The Rpf protein also has weak proteolytic activity against N-CBZ-Gly-Gly-Arg-beta-naphthylamide, a substrate for trypsin-like enzymes. Rpf activity towards 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside was reduced when the glutamate residue at position 54, invariant for all Rpf family proteins and presumably involved in catalysis, was altered. The same amino acid substitution resulted in impaired resuscitation activity of Rpf. The data indicate that Rpf is a peptidoglycan-hydrolyzing enzyme...Continue Reading

References

Oct 1, 1995·Archives of Microbiology·J V Höltje
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·G V MukamolovaD B Kell
Mar 17, 2000·Biochimica Et Biophysica Acta·L L ZavalovaE D Sverdlov
Jan 4, 2001·Journal of Molecular Evolution·N T Blackburn, A J Clarke
Nov 2, 2002·Molecular Microbiology·Galina V MukamolovaMichael Young
Nov 2, 2002·Molecular Microbiology·Galina V MukamolovaMichael Young
Apr 16, 2003·Biochemistry. Biokhimii︠a︡·V M GovorunA I Archakov
Jul 23, 2003·Infection and Immunity·Vladimir V YeremeevAlexander S Apt
Oct 3, 2003·Cellular and Molecular Life Sciences : CMLS·K TakeshitaT Imoto
Jan 20, 2004·Trends in Biochemical Sciences·Martin Cohen-GonsaudGilles Labesse
Feb 22, 2005·Nature Structural & Molecular Biology·Martin Cohen-GonsaudNicholas H Keep
Apr 2, 2005·Protein Science : a Publication of the Protein Society·Jimin Pei, Nick V Grishin
Jan 1, 1972·Methods in Enzymology·W R Gray

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Citations

Aug 7, 2012·Antonie van Leeuwenhoek·Vadim D NikitushkinArseny S Kaprelyants
Oct 3, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Alessia RuggieroRita Berisio
Dec 2, 2009·Journal of Bacteriology·Michael YoungCharles L Greenblatt
Oct 31, 2009·American Journal of Respiratory and Critical Care Medicine·Galina V MukamolovaMichael R Barer
Jan 12, 2013·Current Protein & Peptide Science·Alessia RuggieroRita Berisio
Mar 4, 2014·American Journal of Respiratory and Critical Care Medicine·Wei HuangJijia Shen
Jul 16, 2013·Critical Reviews in Microbiology·Daniela PintoLélia Chambel
Nov 11, 2008·Journal of Molecular Biology·Alessia RuggieroRita Berisio
May 17, 2013·Microbiology·Daniela PintoLélia Chambel
Feb 17, 2015·Molecular Microbiology·Renée J St-OngeMarie A Elliot
May 2, 2012·RNA Biology·Kristine Arnvig, Douglas Young
Jun 12, 2014·Frontiers in Microbiology·Laam LiSebastien P Faucher
Oct 6, 2009·FEMS Immunology and Medical Microbiology·Bavesh Davandra Kana, Valerie Mizrahi
Nov 29, 2017·Frontiers in Microbiology·Jan BobekMatouš Čihák
Jul 29, 2015·Journal of Bacteriology·Natalia Tschowri
Aug 21, 2019·BMC Genomics·Izabela Coimbra IbraimVasco Azevedo
Sep 20, 2019·Microbiology·Andreas LatoschaNatalia Tschowri
May 9, 2007·The Journal of Biological Chemistry·Patrick VeigaSaulius Kulakauskas
Apr 8, 2015·The FEBS Journal·Vadim D NikitushkinArseny S Kaprelyants
Aug 2, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Francois Xavier ChauviacMartin Cohen-Gonsaud
Apr 14, 2018·BMC Microbiology·Iria UhíaBrian D Robertson
Nov 4, 2017·Annals of Clinical Microbiology and Antimicrobials·Galina R DeminaArseny S Kaprelyants
Apr 1, 2014·Microbiology Spectrum·Kristine B ArnvigDouglas B Young

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