PMID: 2498282Jun 1, 1989Paper

Proteins that interact with GTP during sporulation of Bacillus subtilis

Journal of Bacteriology
C Mitchell, J C Vary

Abstract

During sporulation of Bacillus subtilis, several proteins were shown to interact with GTP in specific ways. UV light was used to cross-link [alpha-32P]GTP to proteins in cell extracts at different stages of growth. After electrophoresis, 11 bands of radioactivity were found in vegetative cells, 4 more appeared during sporulation, and only 9 remained in mature spores. Based on the labeling pattern with or without UV light to cross-link either [alpha-32P]GTP or [gamma-32P]GTP, 11 bands of radioactivity were apparent guanine nucleotide-binding proteins, and 5 bands appeared to be phosphorylated and/or guanylated. Similar results were found with Bacillus megaterium. Assuming that GTP might be a type of signal for sporulation, it could interact with and regulate proteins by at least three mechanisms.

References

Jan 18, 1978·Biochimica Et Biophysica Acta·L K Shay, J C Vary
Jan 1, 1988·Annual Review of Microbiology·A J Cozzone
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J AhnnM Inouye
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Feb 1, 1981·Journal of Bacteriology·A H Ullah, G W Ordal

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Citations

May 1, 1992·Molecular Microbiology·P E March
Jan 1, 1991·Applied Biochemistry and Biotechnology·M Starzak, R K Bajpai
Nov 14, 2000·Journal of Bacteriology·Z Prágai, C R Harwood
Nov 1, 1996·Journal of Bacteriology·S M NajafiM D Yudkin

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