ProteinTracker: an application for managing protein production and purification.

BMC Research Notes
Stefan C Ponko, David Bienvenue

Abstract

Laboratories that produce protein reagents for research and development face the challenge of deciding whether to track batch-related data using simple file based storage mechanisms (e.g. spreadsheets and notebooks), or commit the time and effort to install, configure and maintain a more complex laboratory information management system (LIMS). Managing reagent data stored in files is challenging because files are often copied, moved, and reformatted. Furthermore, there is no simple way to query the data if/when questions arise. Commercial LIMS often include additional modules that may be paid for but not actually used, and often require software expertise to truly customize them for a given environment. This web-application allows small to medium-sized protein production groups to track data related to plasmid DNA, conditioned media samples (supes), cell lines used for expression, and purified protein information, including method of purification and quality control results. In addition, a request system was added that includes a means of prioritizing requests to help manage the high demand of protein production resources at most organizations. ProteinTracker makes extensive use of existing open-source libraries and is designed...Continue Reading

References

Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Chris MorrisRobert M Esnouf
Jul 30, 2011·Nature Methods·Marina OlhovskyKaren Colwill

❮ Previous
Next ❯

Citations

Mar 5, 2014·Scientific Reports·Markus ListJan Mollenhauer

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation
affinity purification
transfection

Software Mentioned

Ajax
Servlet
Java
Echo3
Apache Tomcat
HyperText
Hibernate Object Relational Management ( ORM )
Java Servlet
ProteinTracker
Hibernate

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.