PMID: 9540852Apr 16, 1998Paper

Proteoglycan breakdown from bovine nasal cartilage is increased, and from articular cartilage is decreased, by extracellular ATP

Biochimica Et Biophysica Acta
C J BrownD J Buttle

Abstract

The addition of ATP, but not ADP or AMP, to the culture media of bovine nasal cartilage explants caused an acceleration in the rate of proteoglycan loss from the tissue. The ATP-stimulated loss of proteoglycan was not inhibited by the IL1-receptor antagonist protein, but was partially inhibited by the presence of ADP or AMP. The proteolytic events resulting from the presence of ATP were found to be similar to those following treatment with IL1, in that inhibitors of the cysteine-peptidase cathepsin B, serine-proteinases with trypsin-like specificity, and of some of the matrixins, could all prevent proteoglycan loss, which was mediated, at least in part, by the action of 'aggrecanase'. In contrast to its effects on nasal cartilage, ATP inhibited basal and stimulated proteoglycan release from articular cartilage. Both ADP and AMP had no effect on proteoglycan release in articular cartilage but enhanced the response to ATP when added concurrently. We conclude that extracellular ATP, probably acting via P2-purinoceptors, stimulates proteoglycan breakdown from bovine nasal cartilage and thus, may have a role in diseases which primarily involve destruction of non-articular cartilage. Extracellular ATP has, in contrast, a chondroprote...Continue Reading

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Citations

Nov 24, 2004·Cell and Tissue Research·Shimona Gayle, Geoffrey Burnstock
Aug 15, 2013·Purinergic Signalling·Geoffrey BurnstockIsabel R Orriss
Jun 26, 2003·Trends in Pharmacological Sciences·Astrid HoebertzGeoffrey Burnstock
Jan 22, 2013·BMC Complementary and Alternative Medicine·Carla de Campos CicconeMarcelo Augusto Marretto Esquisatto
Oct 15, 2003·Arthritis and Rheumatism·Maryse PicherGreta M Lee
May 17, 2015·Autonomic Neuroscience : Basic & Clinical·Isabel R Orriss
Mar 3, 2010·Tissue Engineering. Part a·Hunter R MoyerZvi Schwartz
Sep 5, 2001·Nature Reviews. Molecular Cell Biology·M YoshidaT Hisabori
May 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A HoebertzT R Arnett
Feb 11, 2020·Frontiers in Pharmacology·Carmen Corciulo, Bruce N Cronstein
Jul 29, 2020·International Journal of Molecular Sciences·Marcin Szustak, Edyta Gendaszewska-Darmach

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