Abstract
Using synovial fluid of individuals with osteoarthritis as a prototypic biologic fluid containing one part proteoglycan per 100 to 1000 parts of other protein, profiles of proteoglycan were produced without preliminary purification through agarose-acrylamide gel electrophoresis, transfer to nitrocellulose, and triple immunoblotting. Chondroitin sulfate, keratan sulfate, and a hyaluronic acid binding region appear to be present on individual synovial fluid proteoglycans in variable amounts, and consequently a triple immunoblot using monoclonal antibodies to these three epitopes has the potential for developing a proteoglycan profile. The profile is assembled by means of densitometric scans of autoradiograms obtained after use of 125I-labeled anti-mouse immunoglobulin. By contrast to the profile of a relatively homogeneous proteoglycan purified from articular cartilage extracts, the proteoglycans of synovial fluid appeared to be quite heterogeneous with the bulk of keratan sulfate epitopes migrating ahead of the bulk of the chondroitin sulfate epitopes. Most of the proteoglycans appeared to possess a hyaluronate binding region.
References
Aug 1, 1976·The Biochemical Journal·P J Roughley, R M Mason
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·W B UpholtA Dorfman
Apr 1, 1977·The Biochemical Journal·V StanescuE Sobczak
Feb 15, 1986·The Biochemical Journal·T T GlantA R Poole
Jun 2, 1986·European Journal of Biochemistry·H MehmetT Feizi
Jul 1, 1986·Arthritis and Rheumatism·T F KresinaR W Moskowitz
May 1, 1987·Arthritis and Rheumatism·J WitterA R Poole
May 1, 1987·Analytical Biochemistry·R Heimer, P M Sampson
Oct 1, 1988·Annals of the Rheumatic Diseases·A RatcliffeT E Hardingham
Sep 1, 1987·Analytical Biochemistry·R HeimerP M Sampson
Dec 1, 1985·Arthritis and Rheumatism·E J ThonarK E Kuettner
Jan 1, 1988·Connective Tissue Research·V StanescuM P Muriel
May 15, 1986·The Biochemical Journal·A R Poole
May 1, 1987·Annals of the Rheumatic Diseases·G J Carroll
Nov 15, 1985·Analytical Biochemistry·D HeinegårdB Larsson
Dec 1, 1971·Analytical Biochemistry·C A McDevitt, H Muir
Jan 1, 1984·Collagen and Related Research·B M Vertel, L L Barkman
Feb 16, 1984·Nature·J R CouchmanJ R Baker
Oct 1, 1984·Cell·Z Avnur, B Geiger
Feb 1, 1981·Collagen and Related Research·S PalA R Poole
Citations
Aug 14, 2003·Scandinavian Journal of Psychology·Lennart Heimer
Jan 1, 1992·Upsala Journal of Medical Sciences·M Wiberg
Jun 1, 1995·Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery·A G LissM Wiberg
Sep 1, 1995·Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery·A G LissM Wiberg
Dec 31, 1990·Biochemical and Biophysical Research Communications·J LakshmananD A Fisher
Mar 21, 1998·Analytical Biochemistry·J MelroseP Ghosh
Sep 1, 1993·Matrix : Collagen and Related Research·R I BasheyR Heimer