PMID: 3753448May 1, 1986Paper

Proteoglycans from pig aorta. Comparative study of their interactions with lipoproteins

The Biochemical Journal
J WegrowskiL Robert

Abstract

Different proteoglycans (PGs) were isolated from pig aorta for aggregation studies with hyaluronic acid and human low-density lipoproteins (LDL). Extraction of the intima-media with 4M-guanidinium chloride and digestion of the residue with collagenase solubilized 91% of aortic hexuronic acid content. From the guanidinium chloride extract two PGs were isolated by ion-exchange and gel-permeation chromatography: proteochondroitin sulphate (PGI) with a protein-core apparent Mr of 250 000 and proteodermatan-chondroitin sulphate (PGII) with a protein-core apparent Mr of 55 000. Only PGI forms high-Mr aggregates with hyaluronic acid. From the collagenase digest two other PGs were isolated: proteoheparan sulphate and proteochondroitin sulphate (PGIII and PGIV respectively). PGIV had a smaller hydrodynamic size than PGI. PGI and PGII formed insoluble complexes with human LDL in the presence of Ca2+. PGIII or PGIV did not form precipitates with the LDL. PGI and PGII, but neither PGIII nor PGIV, were bound to LDL-Sepharose. The main peaks of PGI and PGII were eluted from LDL-Sepharose with 60 mM- and 90 mM-NaCl respectively. The results indicate that aortic PGs have different interacting potentials with lipoproteins, depending on their Mr...Continue Reading

Citations

Jan 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·T N Wight
Jul 31, 1997·Nature·M Gross, K W Plaxco
Mar 21, 1997·The Journal of Biological Chemistry·M O PentikäinenP T Kovanen
Aug 1, 1990·FEBS Letters·J Labat-RobertL Robert
Apr 7, 1993·Biochimica Et Biophysica Acta·M GigliV Rizzo
Jan 1, 1989·The International Journal of Biochemistry·D MargelinJ Picard
Feb 1, 1989·International Journal of Biological Macromolecules·A D CardinD Feldhake
Feb 1, 1990·International Journal of Biological Macromolecules·J Labat-RobertM Bihari-Varga
Jun 1, 1990·International Journal of Biological Macromolecules·J WegrowskiC Dachet

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