PMID: 6402516Jan 1, 1983Paper

Proteoglycans in primate arteries. III. Characterization of the proteoglycans synthesized by arterial smooth muscle cells in culture

The Journal of Cell Biology
T N Wight, V C Hascall

Abstract

Near confluent monolayers of arterial smooth muscle cells derived from Macaca nemestrina were labeled with Na2[35S]O4 and the newly synthesized proteoglycans present in the culture medium and cell layer were extracted with either 4 M guanidine HCl (dissociative solvent) or 0.5 M guanidine HCl (associative solvent) in the presence of protease inhibitors. The proteoglycans in both compartments were further purified by cesium chloride density gradient ultracentrifugation. Two size classes of proteoglycans were observed in the medium as determined by chromatography on Sepharose CL-2B. The large population (Kav = 0.31) contained predominantly chondroitin sulfate chains with Mr = approximately 40,000. The smaller population (Kav = 0.61) contained dermatan sulfate chains of similar Mr (approximately 40,000). When tested for their ability to aggregate, only proteoglycans in the large-sized population were able to aggregate. A chondroitin sulfate containing proteoglycan with identical properties was isolated from the cell layer. In addition, the cell layer contained a dermatan sulfate component which eluted later on Sepharose CL-2B (Kav = 0.78) than the dermatan sulfate proteoglycan present in the medium. Electron microscopy of the puri...Continue Reading

References

Nov 1, 1975·Archives of Biochemistry and Biophysics·K C EhrlichG S Berenson
Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M OkayamaH Holtzer
Jan 1, 1978·Connective Tissue Research·K MadsenA Hinek
Aug 1, 1979·Archives of Biochemistry and Biophysics·K Madsen, S Lohmander
Dec 29, 1978·Biochemical and Biophysical Research Communications·K KimataS Suzuki
Oct 30, 1978·Biochemical and Biophysical Research Communications·B NorlingA Wasteson
Sep 1, 1967·Journal of Atherosclerosis Research·V KumarJ P Strong
May 1, 1970·Comparative Biochemistry and Physiology·V Stefanovich, K Akiyama
Feb 15, 1973·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A GardaisB Hermelin
Aug 1, 1974·Experimental Cell Research·P F GoetinckP D Royal
Mar 1, 1980·Journal of Ultrastructure Research·G K Hascall
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Mangkornkanok-MarkR M Bahu
Aug 1, 1981·The Journal of Cell Biology·J J CastellotM J Karnovsky
Aug 29, 1980·Biochemical and Biophysical Research Communications·S GardellL Rodén
Oct 1, 1960·Proceedings of the Society for Experimental Biology and Medicine·D KAPLAN, K MEYER

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Citations

Jun 1, 1991·Journal of Cellular Physiology·D W Lipke, J R Couchman
Jul 15, 1985·Experientia·A V Somlyo, C Franzini-Armstrong
Apr 1, 1994·Matrix Biology : Journal of the International Society for Matrix Biology·L Y YaoL J Sandell
Jan 1, 1985·Collagen and Related Research·G A PringleT R Mosmann
Feb 1, 1988·Journal of Ultrastructure and Molecular Structure Research·M RichardsonM W Hatton
May 1, 1985·The Journal of Cell Biology·D G PechakA I Caplan
Mar 1, 1986·The Journal of Cell Biology·M G Kinsella, T N Wight
Dec 2, 1985·European Journal of Biochemistry·A Schmidt, E Buddecke
Jul 21, 1999·Journal of Biomechanical Engineering·Y HuangS Weinbaum
Oct 1, 1989·The American Review of Respiratory Disease·T N WightT Aulinskas
Nov 2, 2004·Biological & Pharmaceutical Bulletin·Toshiyuki KajiThomas N Wight
Jan 1, 1985·Connective Tissue Research·M B ContriI P Ronchetti
Jul 8, 2009·Archives of Physiology and Biochemistry·Sundy N Y YangPeter J Little
Oct 1, 1988·Experimental Cell Research·A Schmidt, E Buddecke
Jan 1, 1987·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C R KopmanB Eaton
Nov 1, 1987·Journal of Cellular Physiology·P G McGuireR W Orkin
Aug 7, 2012·The Journal of Toxicological Sciences·Yasuyuki FujiwaraToshiyuki Kaji

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