PMID: 2493651Mar 1, 1989Paper

Proteoglycans synthesized by chondrocytes of human nonarthritic and osteoarthritic cartilage

Proceedings of the Society for Experimental Biology and Medicine
R Shuckett, C J Malemud

Abstract

Chondrocyte cultures were developed from the cell outgrowths of explanted human nonarthritic and osteoarthritic human cartilage. Two significant differences in sulfated proteoglycan synthesis were demonstrated between the chondrocytes obtained in this manner. With 35SO4 to measure newly synthesized proteoglycan, we found that chondrocytes derived from osteoarthritic cartilage secreted significantly less (P less than 0.05) high density proteoglycan into the culture medium than did chondrocytes from nonarthritic cartilage after 20 hr of radiolabeling. This reduced amount of high density proteoglycan was sustained when chondrocytes were maintained in unlabeled culture medium ("chase" medium). In addition, the osteoarthritic chondrocytes secreted an increased amount of low density proteoglycan when compared with their nonarthritic counterparts. The elution profile of secreted high density proteoglycan isolated from the osteoarthritic chondrocyte culture medium was assessed by gel filtration on Sepharose CL-2B and revealed the presence of two proteoglycan subpopulations (Kav, 0.25, 0.58), whereas only one proteoglycan series (Kav, 0.37) was seen in the high density fraction of nonarthritic chondrocyte culture medium. Similar gel fil...Continue Reading

Citations

Jan 1, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Takashi IkenoueR Lane Smith
Dec 1, 1995·Osteoarthritis and Cartilage·C J MalemudT M Haqqi
Jan 1, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·H R LeipoldG Lust
Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·H Savolainen
Dec 1, 1992·Matrix : Collagen and Related Research·C J Malemud, R S Papay

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