PMID: 2116907Jul 12, 1990Paper

Proteoglycans synthesized in vitro by nude and normal mouse peritoneal macrophages

Biochimica Et Biophysica Acta
V L Petricevich, Y M Michelacci

Abstract

Peritoneal macrophages from nude mice were found to be functionally similar to 'activated' macrophages from normal mice. The objective of the present study was to characterize the proteoglycans synthesized and secreted in vitro by peritoneal macrophages isolated from nude and normal Balb/c mice and to investigate the relationship between macrophage 'activation' and changes in the proteoglycan patterns. Macrophages obtained by peritoneal lavage were seeded in Petri dishes. After 2 h incubation at 37 degrees C, the adherent cells (macrophages) were exposed to [35S]sulphate for the biosynthetic labelling of proteoglycans. After incubation, the cell and medium fractions were collected and analysed for proteoglycans and glycosaminoglycans. The glycosaminoglycans were identified and characterized by a combination of agarose gel electrophoresis and enzymatic degradation with specific mucopolysaccharidases. It was shown that 3/4 of the total 35S-labelled glycosaminoglycans were in the extracellular compartment after 24-48 h. The macrophages synthesized dermatan sulphate (68%), chondroitin sulphate (7%) and heparan sulphate (25%). Both cell and medium fractions of normal and nude mouse macrophages contained glycosaminoglycans with the s...Continue Reading

References

Jan 11, 1977·Journal of Chromatography·C P DietrichL O Sampaio
Jun 13, 1975·Annals of the New York Academy of Sciences·M L KarnovskyA Harper
Aug 1, 1978·The Journal of Experimental Medicine·W F Doe, P M Henson
Jan 1, 1989·Comparative Biochemistry and Physiology. A, Comparative Physiology·F C DriessensJ W van Dijk
Jan 1, 1988·Acta histochemica·S O Kolset, T Larsen
Mar 1, 1986·Carbohydrate Research·C A Población, Y M Michelacci
Sep 14, 1986·Biochemical and Biophysical Research Communications·S O Kolset
Feb 20, 1987·Biochimica Et Biophysica Acta·Y M MichelacciC A Población
Sep 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·C P Dietrich, H M De Oca
Nov 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M J Palmoski, P F Goetinck
Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·N Di FerranteR K Berglund
Aug 2, 1967·Journal of the American Chemical Society·G J Thomas, Y Kyogoku
Mar 29, 1983·Biochemical and Biophysical Research Communications·C P DietrichA H Straus
Sep 11, 1980·The New England Journal of Medicine·C F NathanZ A Cohn
Feb 1, 1954·The Journal of Experimental Medicine·R DULBECCO, M VOGT
Jan 1, 1965·The Journal of Experimental Medicine·Z A COHN, B BENSON

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Citations

Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Y M Michelacci, V L Petricevich
Apr 2, 2004·Biochimica Et Biophysica Acta·Daionety A PereiraYara M Michelacci
Apr 18, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·E S SorianoY M Michelacci
Jul 26, 2002·Molecular Microbiology·Gabriele PradelUte Frevert
Dec 1, 1992·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·P D Kittlick, D Engelmann
Dec 17, 2009·Thrombosis Research·M Anna KowalskaMortimer Poncz
Jul 28, 2009·Journal of Thrombosis and Haemostasis : JTH·L RauovaM Poncz
Feb 28, 2013·The FEBS Journal·Martin A ThelinAnders Malmstrom
May 1, 2012·Biochimica Et Biophysica Acta·Cilene Rebouças de LimaYara M Michelacci
Jun 16, 2005·Biochimica Et Biophysica Acta·Cilene R de LimaYara M Michelacci
Aug 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J D LaskinD L Laskin

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