Proteoliposomes in nanobiotechnology

Biophysics Reviews
P CiancagliniR G Stabeli

Abstract

Proteoliposomes are systems that mimic lipid membranes (liposomes) to which a protein has been incorporated or inserted. During the last decade, these systems have gained prominence as tools for biophysical studies on lipid-protein interactions as well as for their biotechnological applications. Proteoliposomes have a major advantage when compared with natural membrane systems, since they can be obtained with a smaller number of lipidic (and protein) components, facilitating the design and interpretation of certain experiments. However, they have the disadvantage of requiring methodological standardization for incorporation of each specific protein, and the need to verify that the reconstitution procedure has yielded the correct orientation of the protein in the proteoliposome system with recovery of its functional activity. In this review, we chose two proteins under study in our laboratory to exemplify the steps necessary for the standardization of the reconstitution of membrane proteins in liposome systems: (1) alkaline phosphatase, a protein with a glycosylphosphatidylinositol anchor, and (2) Na,K-ATPase, an integral membrane protein. In these examples, we focus on the production of the specific proteoliposomes, as well as ...Continue Reading

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Citations

May 16, 2019·Chembiochem : a European Journal of Chemical Biology·Jean-Paul DouliezLaure Béven
Aug 31, 2017·Biophysics Reviews·Maytê BoleanPietro Ciancaglini
Aug 15, 2013·Calcified Tissue International·Ana Maria S SimãoPietro Ciancaglini
May 17, 2017·Biophysics Reviews·Ana P RamosPietro Ciancaglini
Dec 1, 2015·Biophysics Reviews·Ana Maria Sper SimãoPietro Ciancaglini
Nov 25, 2020·Biology·José Edwin Neciosup QuesñayAndre Luis Berteli Ambrosio
Jun 17, 2020·Biotechnology Advances·Binod PrasadMichael Lebert
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