Proteoliposomes with the ability to transport Ca(2+) into the vesicles and hydrolyze phosphosubstrates on their surface

Archives of Biochemistry and Biophysics
Mayte BoleanPietro Ciancaglini

Abstract

We describe the production of stable DPPC and DPPC:DPPS-proteoliposomes harboring annexin V (AnxA5) and tissue-nonspecific alkaline phosphatase (TNAP) and their use to investigate whether the presence of AnxA5 impacts the kinetic parameters for hydrolysis of TNAP substrates at physiological pH. The best catalytic efficiency was achieved in DPPS 10%-proteoliposomes (molar ratio), conditions that also increased the specificity of TNAP hydrolysis of PPi. Melting behavior of liposomes and proteoliposomes was analyzed via differential scanning calorimetry. The presence of 10% DPPS in DPPC-liposomes causes a broadening of the transition peaks, with AnxA5 and TNAP promoting a decrease in ΔH values. AnxA5 was able to mediate Ca(2+)-influx into the DPPC and DPPC:DPPS 10%-vesicles at physiological Ca(2+) concentrations (∼2 mM). This process was not affected by the presence of TNAP in the proteoliposomes. However, AnxA5 significantly affects the hydrolysis of TNAP substrates. Studies with GUVs confirmed the functional reconstitution of AnxA5 in the mimetic systems. These proteoliposomes are useful as mimetics of mineralizing cell-derived matrix vesicles, known to be responsible for the initiation of endochondral ossification, as they succ...Continue Reading

References

Sep 2, 1975·Biochimica Et Biophysica Acta·D PapahadjopoulosT Isac
Jul 1, 1985·Calcified Tissue International·E D Eanes, A W Hailer
Jun 1, 1997·Cellular and Molecular Life Sciences : CMLS·S Liemann, R Huber
Apr 27, 1999·Atherosclerosis·H H Hsu, N P Camacho
Apr 18, 2002·Biochimica Et Biophysica Acta·Frédéric RonzonBernard Roux
Mar 8, 2005·Colloids and Surfaces. B, Biointerfaces·Hérica de Lima SantosPietro Ciancaglini
Apr 20, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Luciano CaseliM Elisabete D Zaniquelli
Jul 31, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Marie-Cécile GiocondiChristian Le Grimellec
Aug 11, 2007·Cell Biology International·Ana Maria S SimãoPietro Ciancaglini
Feb 19, 2008·Journal of Colloid and Interface Science·Luciano CaseliRoger M Leblanc
Aug 15, 2013·Calcified Tissue International·Ana Maria S SimãoPietro Ciancaglini

❮ Previous
Next ❯

Citations

Aug 31, 2017·Biophysics Reviews·Maytê BoleanPietro Ciancaglini
Oct 17, 2018·Journal of Bone and Mineral Metabolism·Ana Maria Sper SimãoPietro Ciancaglini
May 17, 2017·Biophysics Reviews·Ana P RamosPietro Ciancaglini
Feb 23, 2020·International Journal of Molecular Sciences·Ekeveliny Amabile VeschiPietro Ciancaglini
Nov 8, 2017·Biochimica Et Biophysica Acta. General Subjects·Massimo BottiniRene Buchet
May 1, 2021·International Journal of Molecular Sciences·Lukasz BozyckiAgnieszka Strzelecka-Kiliszek
Nov 9, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·R DerradiA P Ramos

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

© 2021 Meta ULC. All rights reserved