Proteolysis in milk during experimental Escherichia coli mastitis

Journal of Dairy Science
F MoussaouiC Burvenich

Abstract

This work consisted of the intramammary infections (IMI) of 8 heifers by high doses of Escherichia coli to study both the proteolytic activity in milk and the resulting peptides. Therefore, a milking kinetic has been followed, and several parameters have been studied, such as proteose peptones (PP) fraction (quantitative and qualitative changes), plasmin activity (PA), milk somatic cell count (SCC), and bacterial count. A qualitative study of milk proteins and PP was performed by sodium dodecyl sulfate-PAGE, and the peptides recovered in PP during the acute phase of inflammation were amino-terminal micro-sequenced. A BSA increase in milk over time supported the hypothesis of an increase in the permeability of the epithelial barrier. A significant increase in PP content, considered to be an indicator of proteolysis, was observed from postinfusion hours (PIH) 12 to 48. Both the E. coli bacterial count and the SCC increased from PIH 3 to 216. Plasmin activity was increased noticeably from PIH 15 to 24. The respective increases in SCC, bacterial count, and PA suggest their involvement in a global mechanism responsible for the increase in proteolysis in milk after E. coli challenge. Somatic cell count and E. coli may be involved fro...Continue Reading

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Citations

Oct 4, 2012·Animal : an International Journal of Animal Bioscience·G LeitnerG Katz
Feb 19, 2013·Animal : an International Journal of Animal Bioscience·M T StumpfP Montagner
Aug 8, 2006·The Journal of Veterinary Medical Science·Yumiko KomineKen-ichi Komine
Jul 28, 2005·Reproduction, Nutrition, Development·Kahina HaddadiYves Le Roux
Sep 6, 2007·Journal of Animal Science·X Zhao, P Lacasse
Jan 1, 2014·Journal of Veterinary Medicine·Mohamed Ezzat AlnakipJorge Barros-Velázquez
Nov 15, 2018·Anais Da Academia Brasileira De Ciências·Ione M P Haygert-VelhoJoão Pedro Velho
Sep 16, 2016·Biological Trace Element Research·Mahavir SinghDinesh Mittal
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Ramūnas AntanaitisAlgimantas Paulauskas

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