PMID: 18205605Jan 22, 2008Paper

Proteolysis of ribosomal protein S1 from Escherichia coli and Thermus thermophilus leads to formation of two different fragments

Biochemistry. Biokhimii︠a︡
O M SelivanovaI N Serduyk

Abstract

As a result of limited tryptic proteolysis of S1 ribosomal protein (molecular mass 60 kD) from Thermus thermophilus, 25 N-terminal amino acid residues and 71 C-terminal amino acid residues are split off and a stable high-molecular-weight fragment with molecular mass of 49 kD is formed that retains RNA-binding properties and is capable of interacting with 30S ribosomal subunit. Earlier, application of a similar procedure for the formation of a fragment of S1 protein from Escherichia coli resulted in splitting of 171 N-terminal amino acid residues with the formation of a 41.3 kD fragment that possesses RNA-binding properties only. Thus, in spite of high homology between E. coli and T. thermophilus proteins, the proteolysis leads to the formation of two different fragments, which points, in our opinion, to the fact of significant differences between their structures.

References

Aug 5, 1978·Journal of Molecular Biology·J O ThomasW Szer
Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A KolbW Szer
Sep 1, 1977·FEBS Letters·L Giri, A R Subramanian
Jan 11, 1991·Nucleic Acids Research·I V BoniN V Tzareva
Jan 1, 1988·Methods in Enzymology·D E DraperJ V Vartikar
Nov 21, 1972·European Journal of Biochemistry·R KamenC Weissmann
Jan 1, 1983·Progress in Nucleic Acid Research and Molecular Biology·A R Subramanian
Aug 1, 1994·Analytical Biochemistry·I D Pokrovskaya, V V Gurevich
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R S CormackG A Mackie
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·J SenguptaJ Frank
Aug 7, 2002·FEBS Letters·Vyacheslav M ShiryaevAlexander S Spirin
Apr 5, 2003·Science·Mikel ValleJoachim Frank
Jul 16, 2003·The Journal of Biological Chemistry·Olga M SelivanovaAlexander S Spirin
Sep 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kathleen E McGinness, Robert T Sauer

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Citations

Jun 23, 2020·Biochemistry. Biokhimii︠a︡·S Yu GrishinO V Galzitskaya

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