Proteolysis of the peanut allergen Ara h 1 by an endogenous aspartic protease

Plant Physiology and Biochemistry : PPB
Karl A. Wilson, A L Tan-Wilson

Abstract

The 7S and 11S globulins of peanuts are subjected to proteolysis two days after seed imbibition, with Ara h 1 and the arachin acidic chains being among the first storage proteins to be mobilized. Proteolytic activity was greatest at pH 2.6-3 and is inhibited by pepstatin A, characteristic of an aspartic protease. This activity persists in seedling cotyledons up to at least 8 days after imbibition. In vitro proteolysis of Ara h 1 at pH 2.6 by extracts of cotyledons from seedlings harvested 24 h after seed imbibition generates newly appearing bands on SDS-PAGE. Partial sequences of Ara h 1 that were obtained through LC-MS/MS analysis of in-gel trypsin digests of those bands, combined with information on fragment size, suggest that proteolysis begins in the region that links the two cupin domains to produce two 33/34 kD fragments, each one encompassing an intact cupin domain. The later appearance of two 18 and 10/11 kD fragments can be explained by proteolysis within an exposed site in the cupin domains of each of the 33/34 kD fragments. The same or similar proteolytic activity was observed in developing seeds, but Ara h 1 remains intact through seed maturation. This is partly explained by the observation that acidification of the...Continue Reading

References

Jan 1, 1978·Journal of Agricultural and Food Chemistry·S M Mahaboob Basha, J P Cherry
Jan 1, 1992·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·P B Szecsi
May 1, 1996·Phytochemistry·A L Tan-WilsonK A Wilson
Sep 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C MillotM Manfait
Sep 28, 1998·Biochimica Et Biophysica Acta·W BleukxJ A Delcour
Jul 6, 2000·Bioscience, Biotechnology, and Biochemistry·H ParkH Kobayashi
May 15, 2002·Biochimica Et Biophysica Acta·Patrick M BoydKarl A Wilson
Mar 20, 2003·Journal of Agricultural and Food Chemistry·Gordana S TimotijevicVesna R Maksimovic
Jan 30, 2004·Acta Crystallographica. Section D, Biological Crystallography·Yukie MaruyamaShigeru Utsumi
May 22, 2004·European Journal of Biochemistry·Isaura Simões, Carlos Faro
Jul 22, 2004·Journal of Agricultural and Food Chemistry·Harry J WichersAart Van Amerongen
Jul 5, 2005·Molecular Nutrition & Food Research·Sander R PiersmaStef J Koppelman
Dec 26, 2006·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·F Javier Moreno
Jan 19, 2007·Journal of Experimental Botany·Fanglian HeAnna Tan-Wilson
Jan 19, 2011·Analytical Biochemistry·Daniel PanAnna Tan-Wilson
Sep 14, 2011·International Archives of Allergy and Immunology·Beatriz CabanillasJesús F Crespo
Sep 16, 2011·The Journal of Biological Chemistry·Maksymilian ChruszczWladek Minor
Oct 25, 2011·Physiologia Plantarum·Anna L Tan-Wilson, Karl A Wilson
May 2, 2012·Allergy, Asthma & Immunology Research·Jihyun KimSang-Il Lee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.