Proteolysis of the urokinase-type plasminogen activator receptor by metalloproteinase-12: implication for angiogenesis in fibrin matrices.

Blood
Pieter KoolwijkVictor W M van Hinsbergh

Abstract

Pericellular proteolysis plays an important role in cell migration and the formation of new capillary structures. The plasminogen activator/plasmin and matrix degrading metalloproteinase (MMP) cascades act together in the remodeling of matrix and cell-matrix contacts. Previously we have shown that the formation of capillary structures by human foreskin microvascular endothelial cells (hMVECs) in a 3-dimensional fibrin matrix requires a functional urokinase-type plasminogen activator receptor (u-PAR). Here we report on the unexpected finding that inhibition of hMVEC-derived MMP activity by BB94 (batimastat) increased the outgrowth of capillary structures in a fibrin matrix. BB94 prevented the release of the u-PA binding domain D1 of u-PAR and thereby increased the number of functional u-PARs on hMVECs without affecting the u-PAR messenger RNA levels. Comparison of various types of protease inhibitors pointed to the prime involvement of MMP activity. Using recombinant MMPs it was shown that MMP-12 activity was able to release the D1 domain of cellularly expressed u-PAR. In addition, the expression of MMP-12 in control and basic fibroblast growth factor/tumor necrosis factor-alpha-stimulated hMVECs was shown by reverse transcripta...Continue Reading

References

Jul 1, 1987·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·V W van HinsberghD C Rijken
May 1, 1982·British Journal of Haematology·G WijngaardsE Groeneveld
Nov 1, 1994·Journal of Vascular Surgery·K M NewmanM D Tilson
Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R M LafrenieF W Orr
Sep 15, 1994·International Journal of Cancer. Journal International Du Cancer·H SolbergG Høyer-Hansen
Apr 1, 1994·The Journal of Clinical Investigation·M R GyetkoR G Sitrin
Nov 1, 1993·The Journal of Clinical Investigation·N PedersenF Jänicke
Aug 1, 1993·The Journal of Cell Biology·M S PepperJ D Vassalli
Feb 1, 1996·The Journal of Clinical Investigation·M DewerchinD Collen
May 1, 1996·Experimental Cell Research·S M KanseK T Preissner
Jul 29, 1996·International Journal of Cancer. Journal International Du Cancer·R H Xing, S A Rabbani
Jan 1, 1996·Enzyme & Protein·P Mignatti, D B Rifkin
Jul 1, 1996·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·H BárdosR Adány
Jan 15, 1997·The Biochemical Journal·N M HooperA J Turner
Jan 15, 1997·European Journal of Biochemistry·G Høyer-HansenK Danø
Jan 27, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T SatoJ M Delaissé
Apr 29, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·T A GiambernardiR J Klebe
May 19, 1998·Pathology, Research and Practice·H WalterA Roessner
Apr 6, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·G MurphyJ Gavrilovic
Apr 6, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·F Blasi
May 4, 1999·The Journal of Clinical Investigation·W G Stetler-Stevenson
Jun 11, 1999·The American Journal of Pathology·M E KroonV W van Hinsbergh

❮ Previous
Next ❯

Citations

Mar 21, 2012·Head and Neck Pathology·Douglas R Gnepp
May 7, 2002·Nature Reviews. Cancer·Mikala Egeblad, Zena Werb
May 2, 2002·Journal of Hematotherapy & Stem Cell Research·Nicanor I Moldovan
Nov 4, 2005·Molecular Biology of the Cell·Roberta MazzieriFrancesco Blasi
Feb 9, 2010·Cardiovascular Research·Helen WilliamsSarah J George
Jan 31, 2012·Cardiovascular Research·Revu Ann AlexanderJohannes M Breuss
Nov 20, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Michael A PortelliIan Sayers
May 1, 2002·Current Opinion in Nephrology and Hypertension·Chris Jackson
Mar 13, 2014·PloS One·Ruipeng Wang, Peter D Sun
Apr 4, 2002·Biological Chemistry·Persio Dello Sbarba, Elisabetta Rovida
Feb 13, 2014·Gene·Alexandre MezentsevSergey Bruskin
May 28, 2011·American Journal of Respiratory Cell and Molecular Biology·Taher NassarAbd Al-Roof Higazi
Jun 25, 2013·Expert Opinion on Investigational Drugs·Domenico Ribatti, Silvia Baiguera
Jun 15, 2007·Critical Reviews in Biochemistry and Molecular Biology·Bénédicte CauweGhislain Opdenakker
Dec 29, 2009·FEBS Letters·Francesco Blasi, Nicolai Sidenius
May 13, 2014·Biochimica Et Biophysica Acta·Yinan Hua, Sreejayan Nair
Jan 14, 2010·Experimental Cell Research·Cyrus M GhajarAndrew J Putnam
Jun 21, 2005·Journal of Cellular and Molecular Medicine·Joyce E Rundhaug
Feb 25, 2005·International Journal of Cancer. Journal International Du Cancer·Madeleine M Handsley, Dylan R Edwards
Nov 5, 2003·The British Journal of Surgery·T A DrixlerI H M Borel Rinkes
Aug 1, 2007·Journal of Thrombosis and Haemostasis : JTH·H R BullerP W Kamphuisen
Nov 11, 2006·Angewandte Chemie·Ivano BertiniKwon Joo Yeo
Mar 8, 2008·International Journal of Cancer. Journal International Du Cancer·Sanaa M NabhaMichael L Cher
Nov 6, 2008·Journal of Thrombosis and Haemostasis : JTH·R T van BeemJ J Zwaginga
Mar 15, 2006·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Caroline CheungBruce M McManus
Jan 3, 2006·Journal of Immunological Methods·Massimo ResnatiFrancesco Blasi
May 24, 2005·Biochimica Et Biophysica Acta·Mikael Björklund, Erkki Koivunen
Mar 13, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Lalita YadavGeetpriya Kaur
Nov 21, 2002·The Journal of Biological Chemistry·Daniela PalmieriPaola Manduca
May 3, 2014·Journal of the American Society of Nephrology : JASN·Dominique CathelinLaurent Mesnard
Dec 13, 2003·Leukemia·M C BénéUNKNOWN EGIL, European Group on Immunological Classification of Leukemias
Aug 19, 2008·Journal of Wound Care·D Stavrou

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.