PMID: 8976194Dec 1, 1996Paper

Proteolytic activation of protein kinase C delta by an ICE/CED 3-like protease induces characteristics of apoptosis

The Journal of Experimental Medicine
T GhayurD Kufe

Abstract

Recent studies have shown that protein kinase C (PKC) delta is proteolytically activated at the onset of apoptosis induced by DNA-damaging agents, tumor necrosis factor, and anti-Fas antibody. However, the relationship of PKC delta cleavage to induction of apoptosis is unknown. The present studies demonstrate that full-length PKC delta is cleaved at DMQD330N to a catalytically active fragment by the cysteine protease CPP32. The results also demonstrate that overexpression of the catalytic kinase fragment in cells is associated with chromatin condensation, nuclear fragmentation, induction of sub-G1 phase DNA and lethality. By contrast, overexpression of full-length PKC delta or a kinase inactive PKC delta fragment had no detectable effect. The findings suggest that proteolytic activation of PKC delta by a CPP32-like protease contributes to phenotypic changes associated with apoptosis.

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Citations

Sep 24, 1999·Journal of Neuroscience Research·S L Chan, M P Mattson
Jul 9, 1998·Pathology Oncology Research : POR·R L RiceJ D Taylor
Aug 25, 2012·Archivum Immunologiae Et Therapiae Experimentalis·Meng ZhaoGuo-Qiang Chen
Jun 9, 2006·Apoptosis : an International Journal on Programmed Cell Death·Ilse S DaehnTimothy E Rayner
Nov 18, 2010·Apoptosis : an International Journal on Programmed Cell Death·Jayaraj RavindranP V Lakshmana Rao
Jan 27, 2007·Digestive Diseases and Sciences·Maria J RedlakThomas A Miller
May 24, 2005·Cancer Letters·Jussi KoivunenJuha Peltonen
Jun 25, 2003·Biochemical and Biophysical Research Communications·Atsushi EnomotoYoshio Hosoi
Jun 12, 2003·European Journal of Pharmacology·Yadavalli RajgopalMohan C Vemuri
Sep 23, 2003·Experimental Cell Research·Dunja LukovicDavid S Ucker
Feb 11, 2003·Pharmacology & Therapeutics·Angela ClerkDonna M Valks
Mar 21, 1998·Journal of Biochemical and Biophysical Methods·B GarroneD Watters
Jan 13, 2000·Trends in Neurosciences·K K Wang
Aug 24, 1999·Neurochemistry International·N Marks, M J Berg
Dec 20, 2000·The Journal of Nutritional Biochemistry·A M PajariM Mutanen
Jul 9, 1999·Bioorganic & Medicinal Chemistry Letters·P A WenderH Ohigashi
Sep 11, 1999·Trends in Cell Biology·A G Porter
Jan 4, 2001·The International Journal of Biochemistry & Cell Biology·I SchmitzP H Krammer
Sep 14, 2004·Nature·Ingrid MecklenbräukerAlexander Tarakhovsky
Jul 4, 1998·Scandinavian Journal of Immunology·V Depraetere, P Golstein
Oct 17, 2009·The Journal of Biological Chemistry·Gry Kalstad LønneChrister Larsson
Mar 14, 2007·The Journal of Biological Chemistry·Shuhua XiaDouglas V Faller
Sep 10, 2009·Antioxidants & Redox Signaling·Margarete LukoszJudith Haendeler

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