Proteolytic activity is involved in changes in intrafollicular insulin-like growth factor-binding protein levels during growth and atresia of ovine ovarian follicles

Endocrinology
N BesnardP Monget

Abstract

In the sheep, follicular growth is characterized by both an increase and a decrease in the level of intrafollicular insulin-like growth factor-binding protein-3 (IGFBP-3) and IGFBPs less than 40 kDa (IGFBP-2, -4, and -5), respectively. In contrast, follicular atresia is associated with a decrease and a large increase in levels of IGFBP-3 and IGFBPs less than 40 kDa, respectively. To assess whether intrafollicular proteases are involved in such changes, follicular fluid from follicles of different sizes and degrees of atresia was incubated alone or with pure human IGFBP-3, -4, or -5 or serum (as a source of exogenous IGFBP-2) for 20 h at 37 C. Samples were then analyzed by Western ligand blotting and by immunoblotting using specific antisera. Ovine follicular fluid from different classes of follicles contained proteolytic activity degrading IGFBP-2, -3, -4, and -5. Degradation of IGFBPs was accompanied by the generation of small proteolytic fragments visualized by immunoblotting or after autoradiography using radiolabeled IGFBP-4. Moreover, follicular growth and atresia were characterized by changes in IGFBP proteolytic activity. Indeed, follicular growth (between 2 and 6 mm in diameter) was characterized by 1) a decrease in IGF...Continue Reading

Citations

Aug 30, 2000·Molecular and Cellular Endocrinology·P Monget, C Bondy
Aug 19, 2003·Reproduction, Fertility, and Development·Jisoo KimSei Kwang Kim
Aug 14, 2002·Reproduction in Domestic Animals = Zuchthygiene·M MihmE J Austin
Apr 26, 2005·FEBS Letters·Pascal FromentJoëlle Dupont
Oct 22, 2008·Molecular Reproduction and Development·Zhilin LiuEric Antoniou
Aug 24, 1999·Endocrine Reviews·L PoretskyL C Giudice
Oct 21, 1999·Molecular Genetics and Metabolism·L A WetterauP Cohen

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