Proteolytic analysis of Trichoderma reesei in celluase-inducing condition reveals a role for trichodermapepsin (TrAsP) in cellulase production

Journal of Industrial Microbiology & Biotechnology
Nayani Dhanushka DaranagamaWataru Ogasawara

Abstract

Filamentous fungi produce a variety of proteases with significant biotechnological potential and show diverse substrate specificities. Proteolytic analysis of the industrial enzyme producer Trichoderma reesei has been sparse. Therefore, we determined the substrate specificity of T. reesei secretome and its main protease Trichodermapepsin (TrAsP) up to P1 position using FRETS-25Xaa-libraries. The role of TrAsP was analyzed using T. reesei QM9414 and the deletant QM∆trasp in Avicel. We observed higher activities of CMCase, Avicelase, and Xylanase in QM∆trasp compared to that of QM9414. Saccharification rate of cellulosic biomass also increased when using secretome of QM∆trasp but the effect was not significant due to the absence of difference in BGL activity compared to QM9414. Higher TrAsP was produced when monosaccharides were used as a carbon source compared to cellulase inducers such as Avicel and α-sophorose. These results elucidate the relationship between TrAsP and cellulase production in T. reesei and suggest a physiological role for TrAsP.

References

Sep 1, 1979·Journal of Bacteriology·D Sternberg, G R Mandels
Jun 1, 1996·Applied and Environmental Microbiology·E Margolles-ClarkM Penttilä
Jul 1, 1997·Trends in Biotechnology·J P van den HomberghJ Visser
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M B RaoV V Deshpande
May 4, 2002·Microbiology·Jesús Delgado-JaranaTahía Benítez
Sep 10, 2003·Biochemical and Biophysical Research Communications·Somporn TanskulKatsumi Takada
Feb 1, 1962·Journal of Bacteriology·M MANDELSE T REESE
Jun 22, 2005·Trends in Biotechnology·K M Helena NevalainenPeter L Bergquist
Mar 10, 2006·Eukaryotic Cell·Roman RauscherRobert L Mach
Apr 20, 2006·Microbiology·Levente KaraffaBernhard Seiboth
Sep 4, 2007·Journal of Biotechnology·Long-Xi YuMaureen R Hanson
Sep 21, 2007·Fungal Genetics and Biology : FG & B·Miguel A Moreno-MateosTahía Benítez
Jan 16, 2013·FEBS Letters·Qing LiBrent L Iverson
Dec 3, 2013·Current Genomics·Antonella AmoreVincenza Faraco
Aug 15, 2015·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Paula Monteiro de SouzaPérola Oliveira Magalhães
May 12, 2016·Journal of Industrial Microbiology & Biotechnology·Ronivaldo Rodrigues da SilvaHamilton Cabral
Sep 1, 2017·Bioengineered·Youssef Ali Abou Hamin NetoHamilton Cabral

❮ Previous
Next ❯

Datasets Mentioned

BETA
AJ276388

Methods Mentioned

BETA
PCR
reverse transcription PCR

Software Mentioned

Avicel
Mascot
imageJ
Excel Solver

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.