Proteolytic bacteria isolated from agro-waste dumpsites produced keratinolytic enzymes

Biotechnology Reports
Nonso E NnolimUchechukwu U Nwodo

Abstract

Microbial bioconversion of carbonoclastic materials is an efficient tool for the exploitation and valorization of underutilized agro-industrial wastes. The agro-industrial sector accumulates tones of keratinous wastes biomass which may be valorized into high value products. Consequently, the keratinolytic potentials of some bacteria isolated from terrestrial milieu was evaluated. Soil samples were collected from dumpsites, keratinase producing bacteria were isolated. Bacterial species were identified through 16S rRNA gene sequences. The keratinase activity was assessed in relation to thiol formation, percentage feather degradation and quantitation of keratinase produced. Keratinolytic bacteria were identified as Bacillus spp. (accession numbers: MG214989 - MG214992, MG214997, MG214998, MG215000, MG215002-MG215005) and Arthrobacter sp. (accession numbers; MG215001). The degree of chicken feather degradation ranged from 61.5 ± 0.71 % to 85.0 ± 1.41 %. Similarly, the activity of keratinase, total protein and thiol group ranged from 198.18 ± 15.43-731.83 ± 14.14 U/mL; 0.09 ± 0.01-0.87 ± 0.05 mg/mL; and 0.69 ± 0.12-2.89 ± 0.11 mM respectively. Notably, Bacillus sp. Nnolim-K1 displayed the best keratinolytic potential with extracellu...Continue Reading

Citations

Jan 8, 2021·AMB Express·Nonso E Nnolim, Uchechukwu U Nwodo
Jan 5, 2021·Frontiers in Microbiology·Nonso E NnolimUchechukwu U Nwodo
Jul 13, 2021·Frontiers in Microbiology·Qingxin Li
Oct 15, 2021·Applied Biochemistry and Biotechnology·Prashant S AryaVikram H Raval

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Datasets Mentioned

BETA
MG214989

Software Mentioned

Basic Local Alignment Search Tool ( BLAST )
BLASTn
BLAST
molecular evolutionary genetics analysis ( MEGA )

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