PMID: 9003424Jan 1, 1997Paper

Proteolytic cleavage of cellubrevin and vesicle-associated membrane protein (VAMP) by tetanus toxin does not impair insulin-stimulated glucose transport or GLUT4 translocation in rat adipocytes

The Biochemical Journal
E HajduchH S Hundal

Abstract

Acute insulin stimulation of glucose transport in fat and skeletal muscle occurs principally as a result of the hormonal induced translocation of the GLUT4 glucose transporter from intracellular vesicular stores to the plasma membrane. The precise mechanisms governing the fusion of GLUT4 vesicles with the plasma membrane are very poorly understood at present but may share some similarities with synaptic vesicle fusion, as vesicle-associated membrane protein (VAMP) and cellubrevin, two proteins implicated in the process of membrane fusion, are resident in GLUT4-containing vesicles isolated from rat and murine 3T3-L1 adipocytes respectively. In this study we show that proteolysis of both cellubrevin and VAMP, induced by electroporation of isolated rat adipocytes with tetanus toxin, does not impair insulin-stimulated glucose transport or GLUT4 translocation. The hormone was found to stimulate glucose uptake by approx. 16-fold in freshly isolated rat adipocytes. After a single electroporating pulse, the ability of insulin to activate glucose uptake was lowered, but the observed stimulation was nevertheless nearly 5-fold higher than the basal rate of glucose uptake. Electroporation of adipocytes with 600 nM tetanus toxin resulted in...Continue Reading

Citations

Sep 26, 2000·American Journal of Physiology. Cell Physiology·L J Foster, A Klip
Jan 17, 2003·Molecular Biology of the Cell·Tatsuru TogoRichard A Steinhardt
Sep 13, 2016·Cellular & Molecular Immunology·Laura Gómez-JaramilloAntonio Campos-Caro
Jan 14, 2011·Traffic·Nia J Bryant, Gwyn W Gould
Feb 13, 2001·American Journal of Physiology. Endocrinology and Metabolism·J W RitchieP M Taylor
Dec 17, 2014·Molecular Biology of the Cell·Jessica B A SadlerGwyn W Gould
Jun 29, 2011·The Journal of Biological Chemistry·Mariela MendezOscar A Carretero
Sep 18, 2009·Journal of Cell Science·Ping ZhaoDavid E James
Nov 5, 2020·Cell Reports·Chandramohan ChitrajuTobias C Walther

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