Proteolytic fragmentation and sugar chains of plasma ADAMTS13 purified by a conformation-dependent monoclonal antibody

Journal of Biochemistry
Hisahide HiuraYoshihiro Fujimura

Abstract

ADAMTS13 is a metalloproteinase that specifically cleaves unusually large von Willbrand factor multimers under high-shear stress. Deficiency of ADAMTS13 activity induces a life-threatening generalized disease, thrombotic thrombocytopenic purpura. We established a simple and efficient method to purify plasma ADAMTS13 (pADAMTS13) from cryosupernatant using an anti-ADAMTS13 monoclonal antibody (A10) that recognizes a conformational epitope within the disintegrin-like domain. Using the purified pADAMTS13, the amino acid residues involved in cleavage by thrombin, plasmin and leucocyte elastase were determined, and the carbohydrate moieties of this enzyme was analysed by lectin blots. Purified pADAMTS13 had a specific activity of 300 U/mg (25,057-fold purification) and the pI was 5.1-5.5. Cleavage sites of the purified pADAMTS13 by three proteases were identified; thrombin cleaved the four peptidyl bonds between Arg257-Ala258, Arg459-Ser460, Arg888-Thr889 and Arg1176-Arg1177, plasmin cleaved the three peptidyl bonds between Arg257-Ala258, Arg888-Thr889 and Arg1176-Arg1177, and elastase cleaved the two peptidyl bonds between Ile380-Ala381 and Thr874-Ser875. Lectin blot analysis indicated the presence of non-reducing terminal α2-6 and ...Continue Reading

Citations

Mar 16, 2013·International Journal of Hematology·Yoshiaki ChinenMasafumi Taniwaki
Jun 12, 2012·Journal of Biochemistry·Yongchol ShinToshiyuki Miyata
May 4, 2011·Journal of Thrombosis and Haemostasis : JTH·K KokameT Miyata
Dec 17, 2014·Proceedings of the National Academy of Sciences of the United States of America·Joshua MuiaJ Evan Sadler
Aug 31, 2016·Blood·Fabian C VerbijJan Voorberg

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