Proteolytic inactivation of cytokines by Pseudomonas aeruginosa.

Infection and Immunity
M J ParmelyW W Zhou

Abstract

Pseudomonas aeruginosa alkaline protease and elastase are thought to contribute to bacterial invasiveness, tissue damage, and immune suppression in animals and patients infected with the bacterium. This study examined the ability of the two proteases to inactivate a number of cytokines that mediate immune and inflammatory responses. Human recombinant gamma interferon (rIFN-gamma) and human recombinant tumor necrosis factor alpha were inactivated by both proteases. Murine rIFN-gamma was relatively resistant to alkaline protease but was inactivated by elastase, and human recombinant interleukin-1 alpha and recombinant interleukin-1 beta were resistant to the effects of both proteases. Western immunoblots suggested that cytokine inactivation by these proteases, where it occurred, required only limited proteolysis of the polypeptides. The ability of different P. aeruginosa strains to inactivate IFN-gamma appeared to require the production of both proteases for optimum activity. These results indicate that in vitro cytokine inactivation by Pseudomonas proteases is selective, requires only limited proteolysis, and in certain instances reflects the cooperative effects of both proteases.

References

Jan 1, 1989·Advances in Immunology·C A Dinarello
Jan 1, 1989·Annual Review of Immunology·B Beutler, A Cerami
Sep 1, 1987·The Journal of Experimental Medicine·J H KehrlA S Fauci
Jul 1, 1987·Scandinavian Journal of Immunology·B K PedersenK Bendtzen
Sep 1, 1987·Pediatric Pulmonology·M J ThomassenC F Doershuk
Jun 1, 1986·The Journal of Experimental Medicine·C A DinarelloJ V O'Connor
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E A Kurt-JonesE R Unanue
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N A Buchmeier, R D Schreiber
Aug 1, 1968·Clinica Chimica Acta; International Journal of Clinical Chemistry·H RinderknechtB J Haverback
Apr 1, 1966·Archives of Biochemistry and Biophysics·K Morihara, H Tsuzuki
Jan 1, 1984·Reviews of Infectious Diseases·C A Dinarello
Sep 1, 1981·The Journal of Experimental Medicine·M Kawakami, A Cerami

❮ Previous
Next ❯

Citations

May 1, 1995·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·B Henderson, M Wilson
Jan 1, 1995·Critical Reviews in Microbiology·H E Duncan, S C Edberg
Sep 1, 1993·The American Review of Respiratory Disease·A Buret, A W Cripps
Aug 9, 2002·DNA and Cell Biology·Jeffery A Hobden
Jul 23, 2005·American Journal of Respiratory Cell and Molecular Biology·Thomas M KrunkoskyJames Travis
Sep 9, 2011·PLoS Pathogens·Bart W BardoelJos A G van Strijp
Sep 24, 2011·Human Vaccines·Anurag SharmaStefan Worgall
Aug 4, 2012·The Journal of Biological Chemistry·Michael B ButterworthPatrick H Thibodeau
Jun 2, 2020·Frontiers in Immunology·Nastaran Mues, Hong Wei Chu
Jun 1, 1995·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·C Duval-JobeM J Parmely
Sep 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Manabu KinoshitaShuhji Seki
Dec 2, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alexander J LaarmanSuzan H M Rooijakkers
Jun 26, 2012·Cell and Tissue Research·P H Thibodeau, M B Butterworth
Apr 24, 2020·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Jimyeong HaKyoung-Hee Choi
Jan 1, 1993·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·C W Czarniecki, G Sonnenfeld
Dec 1, 1993·Journal of Interferon Research·M J ParmelyW W Zhou
Sep 21, 2004·Shock·Pierre Moine, Edward Abraham
May 29, 2003·Critical Care Medicine·Chang-Yi LinArthur S Slutsky
Apr 20, 2004·Pediatric Pulmonology·Marianne S MuhlebachTerry L Noah
Apr 25, 2007·Current Eye Research·Brett A ThibodeauxRichard J O'Callaghan
Dec 4, 2004·Biological Chemistry·Koki Matsumoto
Feb 1, 1992·Journal of Paediatrics and Child Health·K Grimwood
Jan 1, 1997·Journal of Periodontal Research·J FletcherM Wilson
May 1, 2001·Clinical and Diagnostic Laboratory Immunology·S R PetermannL Rust
Mar 25, 2014·Molecular Plant-microbe Interactions : MPMI·Michiel J C PelCorné M J Pieterse
Jul 16, 2003·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Kevin G LeidalGerene M Denning
Nov 1, 2011·Journal of Molecular Recognition : JMR·Bart W Bardoel, Jos A G Strijp

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.