PMID: 6161366Nov 1, 1980Paper

Proteolytic inactivation of luciferases from three species of luminous marine bacteria, Beneckea harveyi, Photobacterium fischeri, and Photobacterium phosphoreum: evidence of a conserved structural feature

Proceedings of the National Academy of Sciences of the United States of America
T F Holzman, Thomas O Baldwin

Abstract

Upon limited proteolysis of luciferases from the luminous marine bacteria Photobacterium fischeri, Photobacterium phosphoreum, and Beneckea harveyi, the rate of loss of luciferase activity is the same as the rate of loss of the heavier subunit of all three enzymes. It thus appears that the larger subunit of the luciferase from P. phosphoreum should be designated alpha based on its apparent homology with the alpha subunits of the luciferases from B. harveyi and P. fischeri. The luciferase from B. harveyi is more sensitive to chymotrypsin than to trypsin; the luciferases of the Photobacterium species are more sensitive to trypsin than to chymotrypsin. Proteolytic inactivation of all three luciferases results from hydrolysis of a few peptide bonds in the alpha subunit; the proteolytic fragments from the three luciferases in 0.50 M phosphate are approximately the same size, indicating that the three enzymes have a protease-labile region at about the same position in the primary structure of their alpha subunits. Phosphate stabilizes all three luciferases against inactivation by proteases. Formation and degradation of intermediate species derived from the alpha subunits are readily observable in all three luciferases. Phosphate alte...Continue Reading

References

Jan 1, 1977·Annual Review of Microbiology·J W Hastings, K H Nealson
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T O BaldwinD A Powers
Feb 4, 1977·Archives of Microbiology·K H Nealson, J W Hastings
Apr 22, 1977·Science·E G Ruby, K H Nealson
Sep 1, 1974·Analytical Biochemistry·D NjusJ W Hastings
Dec 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·J Friedland, J W Hastings
Dec 1, 1969·Biochemistry·J W HastingsA Gunsalus
Dec 8, 1970·Biochemistry·E A MeighenJ W Hastings
Dec 1, 1959·Canadian Journal of Biochemistry and Physiology·B C HUMMEL
Nov 30, 1967·Biochemical and Biophysical Research Communications·T Chase, E Shaw

❮ Previous
Next ❯

Citations

Dec 1, 1995·Current Opinion in Structural Biology·T O BaldwinI Rayment
Aug 28, 2009·The Journal of Biological Chemistry·Zachary T Campbell, Thomas O Baldwin
Dec 16, 2010·Biophysical Journal·Zachary T CampbellOsamu Miyashita
Sep 6, 1996·The Journal of Biological Chemistry·A J FisherI Rayment
Jun 1, 1981·Journal of Bacteriology·C A Reeve, T O Baldwin
Mar 3, 2010·Archives of Biochemistry and Biophysics·Holly R Ellis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.