Abstract
When leupeptin, a thiol protease inhibitor of microbial origin, was injected into rats, the activity of fructose-1,6-bisphosphate aldolase (D-fructose-1,6-bisphosphate D-glyceraldehyde-3-phosphate-lyase, EC 4.1.2.13) in the liver decreased to about 60% of that in control rats. However, the concentration of aldolase protein in the liver extracts, measured with a specific antibody obtained with enzyme purified on a phosphocellulose column, remained unchanged. Injection of leupeptin also caused a marked increase in the activities of free lysosomal proteases, such as cathepsin B (EC 3.4.22.1), cathepsin L (EC 3.4.22.-), cathepsin D (EC 3.4.23.5) and lysosomal carboxypeptidase A in the cytosol fraction. A clear inverse relationship between aldolase and cathepsin B activities in the cytosol fraction was demonstrated. The possibility that the less active form of aldolase detected in the livers of leupeptin-treated rats was produced during homogenization was excluded by showing that the aldolase activity was not changed by addition of various protease inhibitors to the homogenization medium., When insulin was coinjected with leupeptin, increase in the activity of free cathepsin L and decrease of activity of aldolase produced by the inj...Continue Reading
References
Oct 27, 1975·Biochemical and Biophysical Research Communications·U N WiesmannN N Herschkowitz
Apr 1, 1977·European Journal of Biochemistry·H KirschkeP Bohley
Sep 1, 1978·Journal of Biochemistry·T TowatariN Katunuma
Dec 1, 1979·European Journal of Biochemistry·T TowatariN Katunuma
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S PontremoliB L Horecker
Jan 1, 1976·Biochimie·S PontremoliB Poole
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S PontremoliB L Horecker
Apr 2, 1979·European Journal of Biochemistry·P O SeglenA E Solheim
Nov 14, 1979·Biochemical and Biophysical Research Communications·K TanakaA Ichihara
Feb 3, 1978·Science·P Libby, A L Goldberg
Aug 14, 1978·Biochemical and Biophysical Research Communications·N NakaiJ Hase
May 15, 1977·The Biochemical Journal·M F HopgoodF J Ballard
Sep 1, 1974·Journal of Biochemistry·K Matsuda, E Misaka
May 1, 1967·The Journal of Cell Biology·R L Deter, C De Duve
Nov 1, 1974·The Journal of Cell Biology·M Wibo, B Poole
Jul 1, 1967·Journal of Biochemistry·T TanakaH Morimura
Aug 15, 1970·Nature·U K Laemmli
Apr 14, 1980·Biochemical and Biophysical Research Communications·E KominamiN Katunuma
Citations
Jan 1, 1989·The International Journal of Biochemistry·H NakaoH Ogawa
Mar 1, 1991·Mechanisms of Ageing and Development·G O IvyK Kitani
Jan 1, 1982·Advances in Enzyme Regulation·N KatunumaN Wakamatsu
Jan 1, 1992·Archives of Gerontology and Geriatrics·K KitaniG O Ivy
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·H NakaoH Ogawa
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S PontremoliB L Horecker
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A Z ReznickD Gershon
Aug 5, 1991·FEBS Letters·E KominamiN Katunuma
Mar 14, 2007·FEBS Letters·Marek ZarzyckiAndrzej Dzugaj
Mar 27, 1985·Biochimica Et Biophysica Acta·F M van 't HooftA van Tol
Jun 15, 1981·Biochimica Et Biophysica Acta·E KominamiN Katunuma