PMID: 7020765Jun 15, 1981Paper

Proteolytic modification of rat liver fructose-1,6-bisphosphate aldolase by administration of leupeptin in vivo

Biochimica Et Biophysica Acta
E KominamiN Katunuma

Abstract

When leupeptin, a thiol protease inhibitor of microbial origin, was injected into rats, the activity of fructose-1,6-bisphosphate aldolase (D-fructose-1,6-bisphosphate D-glyceraldehyde-3-phosphate-lyase, EC 4.1.2.13) in the liver decreased to about 60% of that in control rats. However, the concentration of aldolase protein in the liver extracts, measured with a specific antibody obtained with enzyme purified on a phosphocellulose column, remained unchanged. Injection of leupeptin also caused a marked increase in the activities of free lysosomal proteases, such as cathepsin B (EC 3.4.22.1), cathepsin L (EC 3.4.22.-), cathepsin D (EC 3.4.23.5) and lysosomal carboxypeptidase A in the cytosol fraction. A clear inverse relationship between aldolase and cathepsin B activities in the cytosol fraction was demonstrated. The possibility that the less active form of aldolase detected in the livers of leupeptin-treated rats was produced during homogenization was excluded by showing that the aldolase activity was not changed by addition of various protease inhibitors to the homogenization medium., When insulin was coinjected with leupeptin, increase in the activity of free cathepsin L and decrease of activity of aldolase produced by the inj...Continue Reading

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Citations

Jan 1, 1989·The International Journal of Biochemistry·H NakaoH Ogawa
Mar 1, 1991·Mechanisms of Ageing and Development·G O IvyK Kitani
Jan 1, 1982·Advances in Enzyme Regulation·N KatunumaN Wakamatsu
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·H NakaoH Ogawa
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S PontremoliB L Horecker
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A Z ReznickD Gershon
Jun 15, 1981·Biochimica Et Biophysica Acta·E KominamiN Katunuma

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