PMID: 3754158Apr 11, 1986Paper

Proteolytic modification of rat prolactin by subcellular fractions of the lactating rat mammary gland

Biochimica Et Biophysica Acta
V L WongR J Witorsch

Abstract

The current study explored prolactin proteolysis by rat lactating mammary gland. 125I-labelled rat prolactin was incubated with tissue fractions of lactating mammary gland and the extent of prolactin degradation and fragment formation was visualized and densitometrically quantitated from autoradiographs derived from SDS-polyacrylamide gel electrophoresis under reducing conditions. At pH 4.5, the 25 000 X g pellet of mammary gland converted intact prolactin (23 kDa band) to proteolytic fragments (8-16 kDa bands) in a time- and tissue concentration-dependent fashion similar to that reported previously for rat ventral prostate. The prolactin-degrading and -fragmenting activity in lactating mammary gland was 5-10-times that observed for ventral prostate, the most active male tissue. This activity at acid pH was also demonstrable in other fractions of mammary gland but appeared to predominate in the cytosol. The above activities in mammary gland virtually disappeared at pH 7.4, appeared sensitive to aspartate and sulfhydryl proteinase inhibitors, and insensitive to serine and metalloenzyme proteinase inhibitors. The distribution of this activity could not be correlated with a particular enzyme marker. These characteristics of mammar...Continue Reading

References

Jan 1, 1978·Hormone Research·H Asawaroengchai
Apr 9, 1981·The New England Journal of Medicine·D T Krieger, J B Martin
Jan 1, 1983·The Prostate·M M Compton, R J Witorsch
Aug 29, 1980·Biochemical and Biophysical Research Communications·I Mittra
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER

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Citations

Apr 18, 2003·Protein Expression and Purification·Matthew GalfioneSue-Hwa Lin
Apr 19, 2008·Endocrinology·Roberta CastinoMichèle Ollivier-Bousquet
Apr 22, 1991·International Journal of Cancer. Journal International Du Cancer·M PlaticaV P Hollander
Aug 28, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jakob TriebelCarmen Clapp
Jan 29, 1988·Biochemical and Biophysical Research Communications·W J DeVito

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