PMID: 9182669Jun 16, 1997Paper

Proteolytic pathways of activation and degradation of a bacterial phospholipase C during intracellular infection by Listeria monocytogenes

The Journal of Cell Biology
Hélène MarquisDaniel A Portnoy

Abstract

Listeria monocytogenes is a facultative intracellular bacterial pathogen that spreads cell to cell without exposure to the extracellular environment. Bacterial cell-to-cell spread is mediated in part by two secreted bacterial phospholipases C (PLC), a broad spectrum PLC (PC-PLC) and a phosphatidylinositolspecific PLC (PI-PLC). PI-PLC is secreted in an active state, whereas PC-PLC is secreted as an inactive proenzyme (proPC-PLC) whose activation is mediated in vitro by an L. monocytogenes metalloprotease (Mpl). Analysis of PI-PLC, PC-PLC, and Mpl single and double mutants revealed that Mpl also plays a role in the spread of an infection, but suggested that proPC-PLC has an Mpl-independent activation pathway. Using biochemical and microscopic approaches, we describe three intracellular proteolytic pathways regulating PCPLC activity. Initially, proPC-PLC secreted in the cytosol of infected cells was rapidly degraded in a proteasome-dependent manner. Later during infection, PCPLC colocalized with bacteria in lysosome-associated membrane protein 1-positive vacuoles. Activation of proPC-PLC in vacuoles was mediated by Mpl and an Mpl-independent pathway, the latter being sensitive to inhibitors of cysteine proteases. Lastly, proPC-PLC...Continue Reading

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Citations

Mar 4, 2000·Advanced Drug Delivery Reviews·C J Provoda, K D Lee
Nov 22, 2002·Microbes and Infection·Howard Goldfine, Sandra J Wadsworth
Mar 6, 1999·Current Opinion in Microbiology·T Hackstadt
Sep 21, 2004·Nature Reviews. Microbiology·Laurent Boyer, Emmanuel Lemichez
Nov 2, 2002·Molecular Microbiology·Martin J StonehouseMichael L Vasil
Jul 4, 2001·Clinical Microbiology Reviews·J A Vázquez-BolandJ Kreft
Jan 17, 2004·Clinical Microbiology Reviews·Herbert Schmidt, Michael Hensel
Apr 16, 2004·Clinical Microbiology Reviews·Douglas A DrevetsRonald A Greenfield
Feb 21, 2002·Infection and Immunity·Lynne M Shetron-RamaNancy E Freitag
Oct 30, 2007·Journal of Bacteriology·Alan Pavinski BitarHélène Marquis
Aug 2, 2011·Journal of Bacteriology·Brian M ForsterHélène Marquis
Apr 16, 2013·Toxins·Sara K B Cassidy, Mary X D O'Riordan
Apr 21, 1999·Molecular Microbiology·D E HigginsD A Portnoy
Sep 24, 1999·The Journal of Cell Biology·J R RobbinsJ A Theriot
Mar 16, 2016·Biochimica Et Biophysica Acta·Qiongying HuangMary F Roberts
Nov 18, 2006·European Journal of Immunology·Francesca SpadaroCarlo Ramoni
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Sep 1, 2008·Microbial Biotechnology·Sukhadeo Barbuddhe, Trinad Chakraborty
Apr 5, 2012·Molecular Microbiology·Brian M Forster, Hélène Marquis
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Mar 4, 2014·Current Opinion in Microbiology·Huan-Yuan ChenFu-Tong Liu
Mar 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Paul Webster

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