PMID: 9450970Apr 4, 1998Paper

Proteolytic processing and Ca2+-binding activity of dense-core vesicle polypeptides in Tetrahymena

Molecular Biology of the Cell
J W Verbsky, A P Turkewitz

Abstract

Formation and discharge of dense-core secretory vesicles depend on controlled rearrangement of the core proteins during their assembly and dispersal. The ciliate Tetrahymena thermophila offers a simple system in which the mechanisms may be studied. Here we show that most of the core consists of a set of polypeptides derived proteolytically from five precursors. These share little overall amino acid identity but are nonetheless predicted to have structural similarity. In addition, sites of proteolytic processing are notably conserved and suggest that specific endoproteases as well as carboxypeptidase are involved in core maturation. In vitro binding studies and sequence analysis suggest that the polypeptides bind calcium in vivo. Core assembly and postexocytic dispersal are compartment-specific events. Two likely regulatory factors are proteolytic processing and exposure to calcium. We asked whether these might directly influence the conformations of core proteins. Results using an in vitro chymotrypsin accessibility assay suggest that these factors can induce sequential structural rearrangements. Such progressive changes in polypeptide folding may underlie the mechanisms of assembly and of rapid postexocytic release. The parall...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 1, 1977·Journal of Ultrastructure Research·R Anderer, K Hausmann
Jan 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C J LumpertH Plattner
Aug 1, 1992·Neuroscience·H Winkler, R Fischer-Colbrie
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M C Yao, C H Yao
Jan 1, 1991·Trends in Biochemical Sciences·W B HuttnerP Rosa
Jan 1, 1989·The Journal of protozoology·D W Martindale
Jul 5, 1985·Journal of Molecular Biology·G von Heijne
Dec 25, 1984·Journal of Molecular Biology·A AdoutteJ Beisson
Oct 15, 1994·Structure·D Baker, D A Agard
Jul 1, 1994·Physiological Reviews·T PozzanJ Meldolesi
Apr 1, 1994·The Biochemical Journal·P A Halban, J C Irminger
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·S Natori, W B Huttner
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·R SweeneyM C Yao
Jan 1, 1997·Neuron·R Rahamimoff, J M Fernandez
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·J C Hutton
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·A Haddad, A P Turkewitz
Nov 1, 1992·Trends in Cell Biology·P Arvan, D Castle

❮ Previous
Next ❯

Citations

Jun 24, 2000·Biochimie·L VayssiéJ Cohen
Nov 29, 2001·Nature Immunology·H R MacDonald, W Held
Nov 6, 2013·The Journal of Cell Biology·Joseph S BriguglioAaron P Turkewitz
Jun 10, 2005·Molecular Biology of the Cell·Alexander J Stemm-WolfMark Winey
Jun 17, 2005·Molecular Biology of the Cell·Andrew T CowanAaron P Turkewitz
Nov 29, 2002·Eukaryotic Cell·Alex HaddadAaron P Turkewitz
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·N D ChilcoatA P Turkewitz
Mar 3, 2007·Trends in Cell Biology·Nels C EldeAaron P Turkewitz
Jul 15, 2005·The Journal of Eukaryotic Microbiology·Grant R BowmanAaron P Turkewitz
Jan 22, 2004·The Journal of Eukaryotic Microbiology·Giovanna Rosati, Letizia Modeo
Mar 19, 2014·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Joseph S Briguglio, Aaron P Turkewitz
Oct 22, 2015·Biological Reviews of the Cambridge Philosophical Society·Helmut Plattner
Oct 30, 2016·Traffic·Sabrice GuerrierAaron P Turkewitz
Nov 19, 2002·The Journal of Biological Chemistry·Niels R BradshawAaron P Turkewitz
Jul 6, 2000·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·B Montdargent, D Letourneur
Feb 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Joseph A KittermanJon Goerke
Jun 19, 2016·G3 : Genes - Genomes - Genetics·Cassandra KonturAaron P Turkewitz
May 29, 2021·Trends in Parasitology·Daniela Sparvoli, Maryse Lebrun

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.