Proteolytic processing and oligomerization of bacteriophage-derived endosialidases

The Journal of Biological Chemistry
Martina MühlenhoffRita Gerardy-Schahn


Bacteriophages infecting the neuroinvasive pathogen Escherichia coli K1 require an endosialidase to penetrate the polysialic acid capsule of the host. Sequence information is available for the endosialidases endoNE, endoNF, and endoN63D of the K1-specific phages phi K1E, phi K1F, and 63D, respectively. The cloned sequences share a highly conserved catalytic domain but differ in the length of the N- and C-terminal parts. Although the expression of active recombinant enzyme succeeded in the case of endoNE, it failed for endoNF. Protein alignments of all three endosialidase sequences gave rise to the assumption that inactivity of the cloned endoNF is caused by a C-terminal truncation. By reinvestigation of the respective gene locus in the phi K1F genome, we identified an extended open reading frame of 3195 bp, encoding a 119-kDa protein. Full-length endoNF contains the C-terminal domain conserved in all endosialidases, which may act as an intramolecular chaperone. Comparative studies carried out with endoNE and endoNF demonstrate that endosialidases are proteolytically processed, releasing the C-terminal domain. Using a mutational approach in combination with protein analytical techniques we demonstrate that (i) the C-terminal dom...Continue Reading


Nov 25, 1988·Nucleic Acids Research·F Corpet
Sep 1, 1986·Journal of Bacteriology·K J Heller, V Krauel
Aug 1, 1982·Journal of Virology·B KwiatkowskiS Stirm
Jun 5, 1995·FEBS Letters·A V KalimanV M Kryukov
Dec 1, 1996·Journal of Bacteriology·R LegouxM Salomé
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Nov 18, 1998·Current Opinion in Structural Biology·Martina MühlenhoffRita Gerardy-Schahn
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·R W HendrixG F Hatfull
Jan 6, 2000·FEMS Microbiology Letters·Y MachidaShinji Iijima
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·D MullerJ Z Kiss
Feb 2, 2002·Nature·Shuji KanamaruMichael G Rossmann


Jan 20, 2004·Journal of Molecular Biology·Dean SchollIan J Molineux
Feb 2, 2010·Nature Structural & Molecular Biology·Eike C SchulzRalf Ficner
Dec 21, 2004·Nature Structural & Molecular Biology·Katharina StummeyerRalf Ficner
Jun 4, 2010·The Journal of Biological Chemistry·James E ThompsonIan S Roberts
Feb 5, 2009·The Journal of Biological Chemistry·David SchwarzerRita Gerardy-Schahn
May 22, 2010·Tissue Engineering. Part a·Kirsten Haastert-TaliniClaudia Grothe
Jan 15, 2008·Journal of Bacteriology·Derek PickardGordon Dougan
Jan 12, 2013·Current Protein & Peptide Science·Zuzanna Drulis-KawaRob Lavigne
Dec 20, 2013·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Jianlong YanJianping Xie
Feb 19, 2011·Current Opinion in Structural Biology·Eike C Schulz, Ralf Ficner
Nov 1, 2008·Current Opinion in Structural Biology·Yu-Jen Chen, Masayori Inouye
May 13, 2006·Molecular Microbiology·Katharina StummeyerMartina Mühlenhoff
Feb 11, 2005·Journal of Molecular Biology·Shuji KanamaruFumio Arisaka
Jan 4, 2005·Journal of Immunological Methods·Anne JokilammiJukka Finne
Mar 30, 2006·Journal of Bioscience and Bioengineering·Yohei KataokaShinji Iijima
Jul 6, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mario Strazzabosco, Luca Fabris
Jan 27, 2017·Journal of Virology·Laura KalinieneRolandas Meškys
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Eike C SchulzRalf Ficner
Mar 25, 2017·Applied Microbiology and Biotechnology·Agnieszka LatkaZuzanna Drulis-Kawa
Dec 5, 2019·Viruses·Carlos G Leon-VelardeMikael Skurnik

Related Concepts

Endo-N-acetylneuraminidase F
Post-Translational Protein Processing
Proteins, Recombinant DNA
Nested Polymerase Chain Reaction
Determination, Sequence Homology
Deletion Mutation
Homologous Sequences, Amino Acid

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.