PMID: 9444982Jan 28, 1998Paper

Proteolytic processing at the amino terminus of human coronavirus 229E gene 1-encoded polyproteins: identification of a papain-like proteinase and its substrate

Journal of Virology
J HeroldS G Siddell

Abstract

Expression of the coronavirus gene 1-encoded polyproteins, pp1a and pp1ab, is linked to a series of proteolytic events involving virus-encoded proteinases. In this study, we used transfection and immunoprecipitation assays to show that the human coronavirus 229E-encoded papain-like cysteine proteinase, PCP1, is responsible for the release of an amino-terminal protein, p9, from the gene 1-encoded polyproteins. The same protein, p9, has also been identified in virus-infected cells. Furthermore, using an in vitro trans-cleavage assay, we defined the proteolytic cleavage site at the carboxyl terminus of p9 as pp1a-pp1ab amino acids Gly-111 and Asn-112. These results and a comparative sequence analysis suggest that substrate positions P1 and P5 seem to be the major determinants of the PCP1 cleavage site and that the latter can occupy a variable position at the amino terminus of the coronavirus ppla and pplab polyproteins. By combining the trans-cleavage assay with deletion mutagenesis, we were also able to locate the boundaries of the active PCP1 domain between pp1a-pp1ab amino acids Gly-861-Glu-975 and Asn-1209-Gln-1285. Finally, codon mutagenesis was used to show that Cys-1054 and His-1205 are essential for PCP1 proteolytic activi...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S Henikoff, J G Henikoff
Jan 1, 1991·Proteins·G D SchulerD J Lipman
Nov 1, 1990·Nature·B MossT R Fuerst
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S Karlin, S F Altschul
May 1, 1990·The Journal of General Virology·T RaabeS G Siddell
Jan 1, 1987·The Journal of General Virology·M E BoursnellM M Binns
Jan 1, 1988·Annual Review of Biochemistry·H G Kräusslich, E Wimmer
Jul 1, 1995·Journal of Virology·J ZiebuhrS G Siddell
Feb 1, 1995·Journal of Virology·S A HughesS R Weiss
Jan 1, 1996·Methods in Enzymology·J HeroldJ Ziebuhr

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Citations

Feb 14, 2002·The Journal of General Virology·Annette Hegyi, John Ziebuhr
Dec 17, 2005·Proteins·Traian SuleaRobert Ménard
Sep 24, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Minsu JangJunsoo Park
Aug 10, 2000·Journal of Virology·A Kanjanahaluethai, S C Baker
Aug 12, 2004·Proceedings of the National Academy of Sciences of the United States of America·Konstantin A IvanovJohn Ziebuhr
Aug 15, 2003·The Journal of General Virology·Volker ThielJohn Ziebuhr
Mar 22, 2000·The Journal of General Virology·J ZiebuhrA E Gorbalenya
Jun 14, 2003·Journal of Virology·Amornrat KanjanahaluethaiSusan C Baker
Feb 16, 2021·Biochemical and Biophysical Research Communications·Minsu JangJunsoo Park
Nov 28, 2021·Scientific Reports·Jéssica de O AraújoAnderson H Lima

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