PMID: 9555075May 23, 1998Paper

Proteolytic processing of a secreted glycoprotein and O-glycosylation of mannoproteins are affected in the N-glycosylation mutant Saccharomyces cerevisiae ldb1

Biochimica Et Biophysica Acta
P MañasL M Hernandez

Abstract

In a previous work [P.I. Mañas, I. Olivero, M. Avalos, L.M. Hernández, Glycobiology, 7 (1997) 487-497], we described the isolation and characterization of the Saccharomyces cerevisiae ldb1 mutant which is affected in several steps of the N-glycosylation of mannoproteins probably due to a malfunction of the Golgi apparatus. Here, we found that two further functions assigned to the Golgi cisternae are also affected in the mutant: proteolytic processing of a secreted protein and O-glycosylation. We found that around 70% of the exoglucanase activity that is secreted into the culture medium by ldb1 bears an extra tetrapeptide in its NH2-terminus due to incomplete proteolytic processing. The O-linked oligosaccharides from ldb1 mnn1 were indistinguishable from those synthesized by the parental strain mnn1. However, when the O-oligosaccharides from the wild type and ldb1 were compared, we found a significant decrease in the tetrasaccharide in the latter, as well as a concomitant increase in the disaccharide, suggesting a defect in the Kre2p/Mnt1p involved in the transfer of the third mannose of these residues.

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A HäuslerP W Robbins
Jan 1, 1991·Methods in Enzymology·Y Kassir, G Simchen
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·L BallouC E Ballou
Nov 1, 1994·The Journal of Cell Biology·E C GaynorS D Emr
Nov 3, 1994·Nature·J E Rothman
Apr 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Herscovics, P Orlean
Mar 1, 1996·The Journal of Cell Biology·S L Harris, M G Waters
Sep 23, 2011·The Journal of Physical Chemistry. a·Buddhadeb GhoshJames B Burkholder
Jun 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bernd K GilsbachArjan Kortholt

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Citations

Apr 20, 2006·Antonie van Leeuwenhoek·Isaac CorbachoLuis M Hernández
Nov 24, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M A Kukuruzinska, K Lennon
Jan 22, 2004·FEMS Yeast Research·Isaac CorbachoLuis M Hernández

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