PMID: 9422384Jan 9, 1998Paper

Proteolytic processing of chromogranin B and secretogranin II by prohormone convertases

Journal of Neurochemistry
A LaslopH Winkler

Abstract

Two experimental approaches were used to study the processing of chromogranin B and secretogranin II by prohormone convertases. In GH3 cells various prohormone convertases were overexpressed together with the substrate chromogranin B by use of a vaccinia virus infection system. PC1 appeared to be by far the most active enzyme and converted chromogranin B to several smaller molecules, including the peptide PE-11. In brain this peptide is cleaved physiologically from chromogranin B. Some processing of chromogranin B and formation of free PE-11 were also observed with PC2 and PACE4. Furin produced larger fragments, whereas PC5-A and PC5-B had negligible effects. As a second model, PC12 cells were stably transfected with PC1 or PC2 to investigate the processing of endogenous chromogranins. Both enzymes effectively cleaved chromogranin B and secretogranin II, liberating the peptides PE-11 and secretoneurin, respectively. However, in transfection experiments the ability to generate the free peptides was more pronounced with PC2 than with PC1. The extent of proprotein processing achieved by prohormone convertases apparently differed depending on the experimental system applied. This suggests that in vivo mechanisms to support and fine...Continue Reading

Citations

Jun 23, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Hidetaka KatoShigeyasu Tanaka
Sep 2, 2005·American Journal of Physiology. Cell Physiology·Sergio Sánchez-ArmássRaul Martínez-Zaguilán
Nov 15, 2006·Annals of the New York Academy of Sciences·Johann GuillemotLaurent Yon
Nov 17, 2007·Acta Physiologica·M Montero-HadjadjeY Anouar
Jun 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Shingo Kurabuchi, Shigeyasu Tanaka
Mar 21, 2003·The New England Journal of Medicine·Laurent TaupenotDaniel T O'Connor
Jun 10, 2004·Molecular Microbiology·Huân M NgôKeith A Joiner
Jun 8, 1999·The Journal of Physiology·J H KoeslagJ A Wessels
May 17, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Margot EggerRudolf Kirchmair
Feb 3, 2005·Biological Reviews of the Cambridge Philosophical Society·Karen B Helle
Jul 23, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Maité Montero-HadjadjeYoussef Anouar
Dec 29, 1998·The Journal of Cell Biology·S UrbéS A Tooze
Jul 23, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Guida Maria Portela-Gomes, Mats Stridsberg
May 13, 1999·Nature Neuroscience·C Stevens
Nov 28, 2008·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Enrico CrivellatoDomenico Ribatti
May 6, 2021·Metabolites·Nicholas NorrisMelkam Alamerew Kebede
Apr 9, 1999·Molecular and Cellular Endocrinology·A M Van Horssen, G J Martens
Mar 1, 2003·Regulatory Peptides·Alfred DoblingerAndrea Laslop
Jun 16, 2009·General and Comparative Endocrinology·E ZhaoVance L Trudeau

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