Proteolytically inactive per a 10 allergen of Periplaneta americana modulates Th2 response and enhances IL-10 in mouse model

Journal of Clinical Immunology
Deepsikha SrivastavaB P Singh

Abstract

Purified allergens with reduced IgE reactivity are required to improve the safety and efficacy of allergen-specific immunotherapy (IT). The present study investigates the efficacy of purified cockroach allergen immunotherapy with proteolytically active and inactive Per a 10 in allergic mouse model. Balb/c mice were sensitized intraperitoneally with cockroach extract (CE) and purified allergen Per a 10 in separate groups. Mice were treated subcutaneously with phosphate-buffered saline (PBS), CE, active and inactive Per a 10 and challenged intranasally. Antigen specific IgE, IgG1 and IgG2a in serum and cytokines IL-4, IL-13, IFN-gamma, IL-10, TGF-beta in bronchoalveolar lavage (BAL) fluid and spleen culture supernatant (CS) were estimated by enzyme-linked immunosorbent assay. Lung histology was analyzed by hematoxylin and eosin staining. IT with Per a 10 demonstrated significant reduction in IgE levels in serum, IL-4 levels in BAL fluid, CS, and eosinophilic infiltration in lungs than PBS-treated mice. This was associated with significantly increased IL-10 secretion in BAL fluid and CS. IT with Per a 10 effectively suppressed T-helper type 2 (Th2) response in mice sensitized with Per a 10 than CE group. Further, IT with inactive ...Continue Reading

References

Feb 1, 1979·The Journal of Allergy and Clinical Immunology·B KangJ W Yunginger
Feb 22, 1996·The New England Journal of Medicine·P J Barnes
Jun 1, 1996·The Journal of Experimental Medicine·M F NeurathW Strober
Jul 3, 1998·The Journal of Clinical Investigation·C A AkdisK Blaser
Nov 5, 1998·The Journal of Allergy and Clinical Immunology·J BousquetH J Malling
Dec 20, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·E M JanssenM H Wauben
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·B G PapouchadoC S David
Jan 16, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Reinhard HillerRudolf Valenta
May 22, 2002·International Archives of Allergy and Immunology·Lili Kazemi-ShiraziRudolf Valenta
Jul 3, 2002·Nature Reviews. Immunology·Rudolf Valenta
Jan 22, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R K BhatM B Hershenson
Feb 6, 2003·The Journal of Clinical Investigation·Jack A EliasZhou Zhu
Mar 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kenji TakabayashiAnthony A Horner
Jun 6, 2003·The Journal of Allergy and Clinical Immunology·James N FrancisStephen R Durham
Apr 15, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G PittnerR Valenta
Jun 23, 2004·The Journal of Allergy and Clinical Immunology·Joost L M VissersAntoon J M van Oosterhout
Sep 26, 2006·Nature Reviews. Immunology·Mark LarchéRudolf Valenta
Oct 31, 2006·Biologicals : Journal of the International Association of Biological Standardization·V Thangam SudhaBhanu P Singh
Feb 15, 2007·Allergy·E D Bateman, A Jithoo
Apr 14, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J BrimnesK Lund
May 29, 2007·The Journal of Allergy and Clinical Immunology·Alain RazafindratsitaPhilippe Moingeon
Jun 29, 2007·The European Respiratory Journal·A K MehtaB P Singh
Aug 12, 2008·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Z ZhangS He
Apr 29, 2009·European Journal of Clinical Investigation·V T SudhaB P Singh

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Citations

Dec 21, 2012·Clinical and Vaccine Immunology : CVI·Dhanapal GovindarajNaveen Arora
Sep 30, 2015·European Journal of Immunology·Komal AgrawalNaveen Arora
Feb 24, 2012·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C GoelN Arora
Aug 22, 2014·Clinical and Experimental Immunology·P SharmaN Arora
Jun 11, 2011·American Journal of Respiratory Cell and Molecular Biology·Vidhu SharmaNaveen Arora
Jan 10, 2019·Infectious Diseases of Poverty·Guang-Li ZhuZhi-Yue Lv

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