Proteome analysis of differentiating human myoblasts by dialysis-assisted two-dimensional gel electrophoresis (DAGE)

Proteomics
Florence GonnetFédor Svinartchouk

Abstract

In the present study, modifications in cytosolic expressed proteins during human myoblast differentiation were studied by dialysis-assisted 2-DE (DAGE, [1]). About 1000 spots were analysed on the 5th and 13th day of differentiation with a dynamic range of protein expression exceeding 1000-fold. During myogenic differentiation, the number of nonmatching spots as well as the extent of quantitative differences between matched spots significantly increased. Over one hundred differentially expressed spots were excised and identified by MALDI-TOF MS. The differentiation-associated expression pattern of eight proteins was validated by Western blot analysis. Differential expression of several proteins was demonstrated for the first time in human myotubes. Interestingly, Ingenuity pathway analysis grouped 30 of these proteins into two overlapping networks containing as principal nodes IGF-1 and tumour necrosis factor, two proteins known to play a crucial role in cytogenesis. Our results illustrate the large rearrangement of the proteome during the differentiation of human myoblasts and provide evidence for new partners involved in this complex process.

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Citations

Jun 20, 2013·Expert Review of Proteomics·Ashling Holland, Kay Ohlendieck
Jan 2, 2013·The Journal of Cell Biology·Evgenia LeikinaLeonid V Chernomordik
Aug 17, 2010·Biochimica Et Biophysica Acta·Kay Ohlendieck
Jan 31, 2009·Proteomics·Philip DoranKay Ohlendieck
Oct 21, 2009·Journal of Cellular and Molecular Medicine·Elise JeanGilles Carnac
Jun 5, 2014·Proteomics. Clinical Applications·Heinrich Brinkmeier, Kay Ohlendieck
Mar 16, 2019·The Journal of Biological Chemistry·Natarajan V BhanuBenjamin A Garcia
Mar 12, 2020·Journal of Cachexia, Sarcopenia and Muscle·Jessy EtienneJean-Thomas Vilquin
Jun 26, 2009·Molecular and Cellular Biochemistry·Hualin SunXiaosong Gu

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