Proteome analysis of digestive fluids in Nepenthes pitchers

Annals of Botany
Sandy RottloffFrederic Bourgaud

Abstract

Carnivorous plants have developed strategies to enable growth in nutrient-poor soils. For the genus Nepenthes, this strategy represents producing pitcher-modified leaves that can trap and digest various prey. These pitchers produce a digestive fluid composed of proteins, including hydrolytic enzymes. The focus of this study was on the identification of these proteins. In order to better characterize and have an overview of these proteins, digestive fluid was sampled from pitchers at different stages of maturity from five species of Nepenthes (N. mirabilis, N. alata, N. sanguinea, N. bicalcarata and N. albomarginata) that vary in their ecological niches and grew under different conditions. Three complementary approaches based on transcriptomic resources, mass spectrometry and in silico analysis were used. This study permitted the identification of 29 proteins excreted in the pitchers. Twenty of these proteins were never reported in Nepenthes previously and included serine carboxypeptidases, α- and β-galactosidases, lipid transfer proteins and esterases/lipases. These 20 proteins display sequence signals allowing their secretion into the pitcher fluid. Nepenthes pitcher plants have evolved an arsenal of enzymes to digest prey cau...Continue Reading

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Citations

May 18, 2016·Archives of Virology·Sissi MiguelAlain Hehn
May 17, 2018·Planta·Michaela SaganováAndrej Pavlovič
Jul 6, 2019·Frontiers in Plant Science·Sissi MiguelFrédéric Bourgaud
Jun 25, 2020·International Journal of Molecular Sciences·Alberto Dávila-LaraAxel Mithöfer
May 24, 2019·Journal of Experimental Botany·Andrej Pavlovič, Axel Mithöfer
Mar 29, 2020·Journal of Experimental Botany·Ondřej KocábAndrej Pavlovič
Jan 30, 2018·Annals of Botany
Apr 24, 2019·Doklady. Biochemistry and Biophysics·M A FilyushinK G Skryabin
May 17, 2017·Proceedings of the National Academy of Sciences of the United States of America·Tianying LanVictor A Albert
Jun 11, 2021·Annals of Botany·Lubomír AdamecAndrej Pavlovič
Jul 21, 2016·Journal of Proteome Research·Linda LeeDavid C Schriemer

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