Proteome analysis of gentisate-induced response in Pseudomonas alcaligenes NCIB 9867

Proteomics
Bing ZhaoChit Laa Poh

Abstract

Pseudomonas alcaligenes NCIB 9867 (P25X wild-type) is capable of degrading aromatic hydrocarbons via the gentisate pathway. Biochemical characterization of P25X mutants indicated that it has isofunctional enzymes for the mono- and dioxygenase-catalyzed reactions. One set of the enzymes is constitutive whereas the other is strictly inducible. To date, only the gene encoding the constitutively-expressed gentisate dioxygenase had been cloned and characterized. A mutant strain of P25X, designated G56, which had the constitutive copy of the gentisate 1,2-dioxygenase gene interrupted by a streptomycin/spectinomycin resistance gene cassette, was found to express gentisate dioxygenase, but only when the cells were induced by gentisate. The proteome profiles of P. alcaligenes P25X and mutant G56 cells grown in the presence and absence of gentisate were compared after two-dimensional polyacrylamide gel electrophoresis. Eight distinctive protein spots (designated M1-M8) which were observed only in induced cells of strain G56 but absent in noninduced cells were further analyzed by matrix-assisted laser desorption/ionization-time of flight, quadrupole-TOF and N-terminal sequencing. Of the 15 proteins (including seven up-regulated) examined,...Continue Reading

Citations

Sep 22, 2007·Omics : a Journal of Integrative Biology·Prashant S PhaleRahul Shrivastava
May 6, 2009·Current Opinion in Microbiology·Seong-Jae KimCarl E Cerniglia
Aug 28, 2007·Research in Microbiology·Chew Chieng YeoChit Laa Poh
Jun 2, 2006·Journal of Microbiological Methods·Wei E HuangJohn Draper
Nov 30, 2006·Journal of Biochemistry and Molecular Biology·Song-Mee BaeKwang-Jun Lee

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