Proteome analysis of human CD56neg NK cells reveals a homogeneous phenotype surprisingly similar to CD56dim NK cells

European Journal of Immunology
Jenny VoigtLothar Jänsch

Abstract

NK cells lacking CD56 (CD56neg ) were first identified in chronic HIV-1 infection. However, CD56neg NK cells also exist in healthy individuals, albeit in significantly lower numbers. Here, we provide an extensive proteomic characterisation of human CD56neg peripheral blood NK cells of healthy donors and compare them to their CD56dim and CD56bright counterparts. Unbiased large-scale surface receptor profiling clustered CD56neg cells as part of the main NK cell compartment and indicated an overall CD56dim -like phenotype. Total proteome analyses of CD56neg NK cells further confirmed their similarity with CD56dim NK cells, and revealed a complete cytolytic inventory with high levels of perforin and granzyme H and M. In the present study, twelve proteins discriminated CD56neg NK cells from CD56dim NK cells with nine up-regulated and three down-regulated proteins in the CD56neg NK cell population. Those proteins were functionally related to lytic granule composition and transport, interaction with the extracellular matrix, DNA transcription or repair, and proliferation. Corroborating these results, CD56neg NK cells showed modest cytotoxicity, degranulation, and IFN-ɣ secretion as compared to CD56dim NK cells. In conclusion, CD56neg ...Continue Reading

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Citations

Apr 10, 2020·Blood Advances·Samantha L SmithDaniel M Davis
Dec 5, 2019·Frontiers in Immunology·Iva FilipovicNiklas K Björkström
Dec 17, 2019·AIDS Research and Human Retroviruses·Netanya S UtayRoberto C Arduino
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Nov 6, 2020·Scandinavian Journal of Immunology·Hans-Gustaf Ljunggren
Jan 8, 2021·Liver International : Official Journal of the International Association for the Study of the Liver·Ratna S WijayaGolo Ahlenstiel
Jan 29, 2021·Clinical & Translational Immunology·Martin R Goodier, Eleanor M Riley
Jun 13, 2021·Nature Reviews. Immunology·Niklas K BjörkströmHans-Gustaf Ljunggren

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