Abstract
Peritoneal endometriosis is the result of ectopic implantation and growth of endometrium tissue that has been regurgitated into the abdominal cavity during menstruation. We have previously shown that menstrual effluent induces epithelial to mesenchymal transitions (EMT) in mesothelial cells, which results in cell retraction and exposure of submesothelial extracellular matrix. Since endometrial tissue preferentially adheres to the extracellular matrix, adhesion of endometrial tissue to the peritoneum is facilitated. The EMT were shown to be associated with differential expression and phosphorylation of mesothelial proteins. Using radiolabeling and proteomics we detected changes in protein expression and phosphorylation that occur in mesothelial cells during the EMT process. The identity of 73 proteins, which were obtained from 324 analyzed spots, was confirmed. The expression of 35 proteins involved in organization of the cytoskeleton, signal transduction, regulation of the redox state, and production of ATP, was altered during the EMT process. Four of the identified proteins were differentially phosphorylated: annexin-1, an actin-binding protein and a substrate for receptor tyrosine kinases; tropomyosin-alpha, a regulator of ac...Continue Reading
Citations
Dec 20, 2008·Human Reproduction·A AmetzazurraD Nagore
Jun 26, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Regina VarghaChristoph Aufricht
Jan 9, 2009·Proteome Science·Jennifer J HillAnne E G Lenferink
Aug 6, 2005·Cell and Tissue Research·Ayşe Y DemirAnton F P M de Goeij
Dec 5, 2014·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Amy K SchuttDale W Stovall
Oct 22, 2008·Expert Review of Proteomics·Simone FerreroJurgis Gedis Grudzinskas
Mar 3, 2015·Journal of Proteome Research·Qian ChenChen Xu
Sep 22, 2007·Fertility and Sterility·Haiyuan LiuShuzhen Wu
Aug 24, 2005·Cancer·Tracey B LewisPhilip S Bernard
Aug 25, 2015·Annals of the New York Academy of Sciences·Tien-Ling LiaoShu-Huei Kao
Jul 13, 2005·Fertility and Sterility·Kathy L Sharpe-Timms
Sep 26, 2013·Clinical Science·Amalia ForteUmberto Galderisi
Jan 25, 2011·Fertility and Sterility·Muna M El-KastiChristian M Becker
May 19, 2018·The Journal of Pathology·Sara NamvarSarah E Herrick
Jan 19, 2019·Oncology Letters·Ping ChenZhigang Huang
Dec 17, 2016·Plant Biology·V PruthviK N Nataraja
Nov 1, 2013·Molecular Medicine Reports·Hiroshi KobayashiHirotaka Kajihara
Aug 5, 2017·Frontiers in Surgery·Ekaterini Christina TampakiGregory Kouraklis
Apr 11, 2006·Journal of Proteome Research·Hong ZhaoXiangdong Wang
Jun 30, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Samira AsghariMehdi Yousefi