Proteome analysis of lipid rafts in Jurkat cells characterizes a raft subset that is involved in NF-kappaB activation

Journal of Proteome Research
Xiaolin TuA E Nel

Abstract

Lipid rafts are detergent-insoluble membrane domains that play a key role in signal transduction by the T-cell antigen receptor. Proteome analysis revealed the presence of amidosulfobetaine-soluble signal transducing, integral membrane, cytoskeletal, heat shock, and GTP-binding proteins in rafts prepared from Jurkat cells. Several of these proteins were recruited to rafts by CD3/CD28 costimulation. Of particular interest is the inducible association of activated IkappaB kinase complexes with raft vesicles that could be captured with anti-flotillin-1 antibodies. Following amidosulfobetaine solubilization, flotillin-beta and IKKbeta underwent reciprocal co-immunoprecipitation. Treatment of Jurkat cells with methyl-beta-cyclodextrin disrupted the assembly and activation of this raft complex and also interfered in CD3/ CD28-induced activation of a NF-kappaB response element in the IL-2 promoter.

References

Jan 28, 1994·Cell·A Weiss, D R Littman
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Aug 1, 1997·Current Opinion in Cell Biology·T Harder, K Simons
Sep 17, 1998·The Journal of Biological Chemistry·E W Harhaj, S C Sun
Dec 16, 1998·Immunological Reviews·J D PowellR H Schwartz
Jan 16, 1999·Molecular and Cellular Biology·F MercurioA M Manning
Feb 16, 1999·Cell·J M Penninger, G R Crabtree
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·J P WhiteleggeH R Kaback
Mar 21, 2000·Seminars in Immunology·P W JanesP S Kabouridis
Apr 12, 2000·Biochemistry·J le CoutreK F Faull
May 12, 2000·European Journal of Biochemistry·M P MolloyA A Gooley
Jun 3, 2000·Annual Review of Immunology·O Acuto, D Cantrell
Apr 20, 2001·Cytokine & Growth Factor Reviews·A Schlegel, M P Lisanti
Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·C Gómez-MóutonC Martínez-A
Dec 12, 2001·American Journal of Physiology. Endocrinology and Metabolism·Perry E Bickel
Jan 29, 2002·European Journal of Immunology·Silvia ValensinCosima T Baldari
Feb 19, 2002·The Plant Journal : for Cell and Molecular Biology·M HipplerP Hoerth
Feb 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew E Schade, Alan D Levine
Feb 28, 2002·Molecular Cell·Guoqing ChenDavid V Goeddel
May 3, 2002·Immunology·Doreen A Cantrell
Aug 19, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Ndaisha SlaughterAndre Nel

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Citations

Sep 10, 2005·Molecular & Cellular Proteomics : MCP·Xiaoning Lu, Haining Zhu
Jan 13, 2009·Journal of Lipid Research·Yu Zi ZhengLeonard J Foster
Jul 7, 2009·Journal of Lipid Research·Yu Zi Zheng, Leonard J Foster
Oct 18, 2008·Journal of Proteomics·Yu Zi Zheng, Leonard J Foster
Nov 3, 2005·The FEBS Journal·Yukiko ShimadaYoshiko Ohno-Iwashita
Jul 26, 2005·Proteomics·Lynne ThadikkaranJean-Daniel Tissot
Jul 20, 2007·Proteomics·Richard R Sprenger, Anton J G Horrevoets
May 10, 2007·Clinical and Experimental Immunology·M M GrantH R Griffiths
Oct 11, 2008·Proteomics·Christian PreisingerWalter Kolch
Oct 1, 2007·Proteomics. Clinical Applications·Aine T BehanPatricia B Maguire
Jul 20, 2007·European Journal of Immunology·Banabihari GiriDennis D Taub
May 9, 2007·Electrophoresis·Michimoto KobayashiSeisuke Hattori
Sep 17, 2011·Journal of Proteomics·Elena Bonzon-KulichenkoJesús Vázquez
Sep 1, 2006·Molecular & Cellular Proteomics : MCP·Petra MannováLaura Beretta
Apr 5, 2016·Pharmacognosy Reviews·Uorakkottil IlyasPunnooth Poonguzi Naseef
Feb 3, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Matthias F LanghorstClaudia A O Stuermer
Jan 9, 2007·The Biochemical Journal·Gonzalo P SolisClaudia A O Stuermer
Oct 26, 2007·Experimental Biology and Medicine·Raewyn C PoulsenMarlena C Kruger
Aug 10, 2020·Journal of Lipid Research·Cerina ChhuonIda Chiara Guerrera

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