Proteome analysis of membrane fractions in colorectal carcinomas by using 2D-DIGE saturation labeling

Journal of Proteome Research
Patricia AlfonsoJ Ignacio Casal

Abstract

Because of its high content in receptors and signaling proteins, the analysis of membrane fractions is critical for the study of neoplastic diseases as colorectal cancer. Here, we have used the new saturation labeling for 2D-DIGE analysis of the membrane proteome of colorectal cancer mucosal tissues. Samples from 6 patients (tumoral and normal paired biopsies) were included in this study. Twelve analytical gels were performed and considered for the quantitative study and statistical analysis. A spot pattern analysis, by using an unsupervised clustering algorithm, allowed the classification of the samples according to similar expression patterns in tumoral and normal samples. Those proteins whose expression changed significantly (Student's t-test, p < 0.05) were further digested and characterized by mass spectrometry. Among the differentially expressed proteins: annexin A2, annexin A4, annexin A5, annexin A7, lamin B, calponin 1 and VDAC were analyzed by immunohistochemistry using tissue microarrays. Annexin A2, annexin A4 and VDAC appear as potential markers of interest for colorectal cancer diagnosis and, presumably, therapy. In summary, saturation labeling provides a new and sensitive tool for the analysis of scarce amounts o...Continue Reading

References

Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M Gonzatti-HacesG F Vande Woude
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
May 6, 1998·Protein Science : a Publication of the Protein Society·E Wallin, G von Heijne
Apr 1, 1999·The New England Journal of Medicine·J S MenellK A Hajjar
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·M SrivastavaH B Pollard
Aug 26, 2000·Progress in Biophysics and Molecular Biology·J I Kourie, H B Wood
Dec 8, 2000·Biochimica Et Biophysica Acta·J H Rand
Aug 16, 2001·Proceedings of the National Academy of Sciences of the United States of America·F M BrichoryS M Hanash
Mar 1, 2003·Nature Biotechnology·Christine C Wu, John R Yates
Nov 16, 2004·Proteomics·Angelika GörgMichael J Dunn
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Volker GerkeStephen E Moss
Jul 26, 2005·Proteomics·Emil SagynalievMarc-André Reymond
May 31, 2006·Molecular & Cellular Proteomics : MCP·Juan Madoz-GúrpideJ Ignacio Casal
Jul 13, 2006·Proteomics·Stefan HellingKatrin Marcus
Oct 5, 2006·Acta Cytologica·Carlos Torres-CabalaMaria J Merino
Oct 13, 2006·Biochimica Et Biophysica Acta·Harold TjalsmaDorine W Swinkels
Oct 25, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Gershon Y LockerUNKNOWN ASCO
Nov 7, 2006·Biochimica Et Biophysica Acta·Paul DowlingMartin Clynes
Feb 24, 2007·Journal of Proteome Research·Sofia BengtssonPeter James
Mar 7, 2007·Journal of Proteome Research·Laura González-SantiagoJ Ignacio Casal
Sep 13, 2007·Molecular & Cellular Proteomics : MCP·Juan Madoz-GúrpideJ Ignacio Casal
Oct 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Michael E HudsonMichael Snyder

❮ Previous
Next ❯

Citations

Jan 26, 2012·European Journal of Endocrinology·Anastasios SofiadisCatharina Larsson
Feb 23, 2011·International Journal of Molecular Sciences·Larissa BelovRichard I Christopherson
Apr 20, 2014·World Journal of Gastroenterology : WJG·Paula Alvarez-ChaverVicenta S Martínez-Zorzano
Aug 19, 2009·Journal of Molecular Neuroscience : MN·Mirele Daiana PoletiMarcelo de Cerqueira César
Mar 20, 2010·Cellular and Molecular Life Sciences : CMLS·Young-Joo JeonKwang-Hee Bae
Dec 5, 2009·Critical Reviews in Clinical Laboratory Sciences·Georgia IkonomouGeorge Panayotou
Feb 1, 2014·Expert Opinion on Therapeutic Targets·Shinya MatsuzakiTetsuji Naka
Aug 3, 2010·Expert Opinion on Therapeutic Targets·Ayako KimTetsuji Naka
Jan 14, 2010·Journal of Biomedicine & Biotechnology·Chantragan SrisomsapJisnuson Svasti
Oct 24, 2013·International Journal of Cancer. Journal International Du Cancer·Shinya MatsuzakiTetsuji Naka
Jun 12, 2009·Electrophoresis·Jonathan S MindenKai Stühler
Oct 7, 2011·The FEBS Journal·Céline HamelinGeneviève Choquet-Kastylevsky
Dec 8, 2010·Proteomics. Clinical Applications·Rodrigo BarderasJosé Ignacio Casal
May 4, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Chunmei GuoMing-Zhong Sun
Jun 7, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Bin WeiMing-Zhong Sun
Jun 7, 2014·Pflügers Archiv : European journal of physiology·Dong LuoMichael Föller
Oct 25, 2017·Expert Review of Proteomics·Abdo Alnabulsi, Graeme I Murray
Feb 18, 2012·Nature Reviews. Cancer·Kin-Hoe ChowKatharine S Ullman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer -Omics

A variety of different high-throughput technologies can be used to identify the complete catalog of changes that characterize the molecular profile of cohorts of tumor samples. Discover the latest insights gained from cancer 'omics' in this feed.