Proteome analysis of multidrug resistance in vincristine-resistant human gastric cancer cell line SGC7901/VCR

Proteomics
Yi-Xuan YangGuo Zhu

Abstract

In order to elucidate the mechanisms of multidrug resistance (MDR) of vincristine-resistant human gastric carcinoma cell line SGC7901/VCR, 2-DE was used to separate the total proteins of SGC7901/VCR and its parental cell line SGC7901. PDQuest software was applied to analyze 2-DE images, and the differential protein spots were identified by both MALDI-TOF-MS and ESI-Q-TOF-MS. Then the differential expressional levels of partially identified proteins were determined by Western blot analysis and real-time RT-PCR. Furthermore, the association of heat shock protein (HSP27), one of the highly expressed proteins in sgc7901/vcr, with MDR was analyzed using antisense inhibition of HSP27. In this study, the well-resolved, reproducible 2-DE patterns of SGC7901/VCR and SGC7901 were established, and yielded about 1100 protein-spots each. All the 24 differential proteins between the two cell lines were identified, and the differential expression levels of the partial proteins were confirmed. The suppression of HSP27 expression by HSP27 antisense oligonucleotides could enhance vincristine chemosensitivity in sgc7901/vcr and induce the cells to exhibit apoptotic morphological features after vincristine treatment. The differentially expressed p...Continue Reading

References

Jun 20, 1998·Archives of Biochemistry and Biophysics·D M GaddeM T McCammon
Oct 7, 1998·International Journal of Cancer. Journal International Du Cancer·L M Vargas-RoigD R Ciocca
Apr 24, 2001·Environmental and Molecular Mutagenesis·A Ronen, B W Glickman
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 29, 2002·Free Radical Biology & Medicine·Su Min LeeJeen-Woo Park
Sep 19, 2002·Glia·Wolfgang Walz
Oct 28, 2003·Hematological Oncology·Brian L Abbott
Dec 30, 2003·International Journal of Cancer. Journal International Du Cancer·Byong Chul YooJae-Gahb Park
Jun 3, 2004·Electrophoresis·Giovanni CandianoPier Giorgio Righetti
Jul 9, 2004·International Journal of Cancer. Journal International Du Cancer·Siri T LehtonenVuokko L Kinnula

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Citations

Mar 3, 2011·Clinical & Experimental Metastasis·Ying-Fu LiuZhu-Chu Chen
Apr 22, 2009·Medical Oncology·Gaoming XiaoZhiqiang Xiao
Jun 16, 2012·Journal of Physiology and Biochemistry·Bei-Bei ZhengXiu-Li Guo
Dec 17, 2009·Omics : a Journal of Integrative Biology·Fengjia ZhuJiang Cao
Dec 12, 2012·BMC Medical Genomics·Jianping LiuZhuchu Chen
Mar 18, 2009·World Journal of Gastroenterology : WJG·Da-Long WuGuo-Jie Song
Jul 14, 2010·Journal of Biomedicine & Biotechnology·Huai-Dong HuSang-Lin Li
Sep 2, 2008·Journal of Cancer Research and Clinical Oncology·Yi-Xuan YangZhi-Qiang Xiao
Aug 1, 2012·Journal of Cancer Research and Clinical Oncology·Bin ZhangXue-Ping Feng
Apr 27, 2012·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Peng KuangYinmin Zhao
May 11, 2012·The International Journal of Biochemistry & Cell Biology·Amina Zoubeidi, Martin Gleave
Apr 14, 2012·Journal of Proteomics·Li-Ling LinHsueh-Fen Juan
Jul 3, 2010·Science China. Life Sciences·Xue GaoFuChu He
Dec 9, 2008·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Huixin YaoZhuchu Chen
Sep 15, 2007·Cancer Treatment Reviews·Jian-Ting Zhang, Yang Liu
Aug 1, 2009·Proteomics. Clinical Applications·Hermann Lage
May 2, 2008·International Journal of Cancer. Journal International Du Cancer·Lin XiaDaiming Fan
Apr 23, 2013·Biochemical and Biophysical Research Communications·Fang WangDaiming Fan
Oct 4, 2011·Journal of Proteomics·Xin-Hui LiZhi-Qiang Xiao
Sep 7, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Fan ZhangXiaoying Wu
Nov 14, 2015·PloS One·Chao WangXiaoyan Zhang
Oct 29, 2010·Acta Biochimica Et Biophysica Sinica·Yaxin ZhaoWei Jin
Jun 20, 2013·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Wanzhong YinWei Zhu
Feb 23, 2007·The Surgeon : Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland·S ChuthapisithO Eremin
Nov 25, 2018·Journal of Cellular Physiology·Atena SoleimaniSeyed Mahdi Hassanian
Oct 1, 2011·Molecular and Cellular Biochemistry·Jin-Jian LuLing-Hua Meng

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