Proteome analysis of rice uppermost internodes at the milky stage

Proteomics
Pingfang YangTingyun Kuang

Abstract

Uppermost internodes, which connect the part between the ear and lower stem, form an important pathway transporting mineral nutrition from roots and photosynthates from leaves (especially the flag leaf) to the ear. The milky stage is the first stage of seed ripening. The uppermost internodes of rice at the milky stage are critical for seed quality and yield. Total soluble proteins of the uppermost internodes of rice (Oryza sativa L. ssp. indica) at the milky stage were analyzed using proteomic methods. Using 2-DE, 762 reproducible protein spots were detected. Among them, 132 abundant proteins were analyzed using MALDI-TOF-MS. Searching in the National Center for Biotechnology Information database, we could identify 98 proteins, which represent 80 gene products. These proteins belong to 11 functional groups with energy production-associated proteins in the first place. The large accumulation of proteins involved in metabolism, signaling, and stress resistance indicated that the uppermost internodes of rice have a high physiological and stress-resistant activity. In addition, our results will also enrich the database of the rice proteome.

References

Jul 21, 1995·The Journal of Biological Chemistry·S R PfefferT Soldati
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D S ConklinD Beach
Mar 1, 1995·Trends in Biochemical Sciences·A Aitken
Dec 1, 1994·Plant Molecular Biology·M S KangM J Cho
May 1, 1994·Electrophoresis·A TsugitaY Nozu
Aug 1, 1994·Plant Physiology·F Van BreusegemA Caplan
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·E A MaherC J Lamb
Dec 16, 1993·Nature·M S Boguski, F McCormick
Jun 27, 1997·Biochimica Et Biophysica Acta·V Gerke, S E Moss
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·S YoshimuraT Sasaki
Aug 29, 1998·The Plant Journal : for Cell and Molecular Biology·I KovácsE C Tóth
Jul 20, 1999·Science·C Somerville, S Somerville
Mar 14, 2000·Current Opinion in Plant Biology·T Sasaki, B Burr
Mar 14, 2000·The Journal of Biological Chemistry·A HofmannR Huber
Apr 1, 2000·The Journal of Biological Chemistry·L CamoniP Aducci
Apr 5, 2000·Biochimica Et Biophysica Acta·M Maeshima
Mar 28, 2002·Physiological Reviews·Volker Gerke, Stephen E Moss
Apr 6, 2002·Science·Stephen A GoffSteven Briggs
Aug 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Antonius KollerJohn R Yates
Oct 3, 2002·Journal of Biochemistry·Shihua ShenSetsuko Komatsu
Feb 26, 2003·Molecular & Cellular Proteomics : MCP·Setsuko KomatsuGuangxiao Yang
May 16, 2003·Proteomics·Tursun KerimBarry G Rolfe
Nov 5, 2003·Electrophoresis·Randeep Rakwal, Ganesh Kumar Agrawal
Mar 23, 2004·Physiologia Plantarum·Robert J. Ferl
May 29, 2004·Planta·Daisuke HoshinoTakahide Tsuchiya
Jul 28, 2004·Phytochemistry·Md Monowar Karim Khan, Setsuko Komatsu
Jul 28, 2004·Phytochemistry·Valérie MéchinCatherine Damerval
Dec 22, 2004·The Plant Journal : for Cell and Molecular Biology·Paul W J van den WijngaardAlbertus H De Boer
Dec 22, 2004·The Plant Journal : for Cell and Molecular Biology·Jan DettmerKarin Schumacher
Jan 27, 2005·Proteomics·Shunping YanWeining Sun

❮ Previous
Next ❯

Citations

Dec 15, 2011·Plant Cell Reports·Sravan Kumar JamiP B Kirti
Dec 7, 2010·Molecular Biotechnology·Raveendran MuthurajanJohn Bennett
Jul 29, 2009·Biochimica Et Biophysica Acta·Feng HanShi-Hua Shen
Jul 27, 2007·Proteomics·Jesús V JorrínMa Angeles Castillejo
Jul 11, 2008·Journal of Separation Science·Wei WangShaoning Chen
Feb 13, 2009·Proteomics·Ganesh Kumar AgrawalRandeep Rakwal
Apr 10, 2013·Genetics and Molecular Biology·Yanhua YangKeping Chen
Dec 17, 2014·Genetics and Molecular Biology·Yanhua YangKeping Chen
Mar 1, 2009·Functional Plant Biology : FPB·Xing Lai PanShi Hua Shen
Jun 3, 2015·Journal of Proteome Research·Subhra ChakrabortySetsuko Komatsu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.