PMID: 15217726DOI: 10.1016/j.ijpara.2004.05.002Jun 26, 2004Paper
Proteome analysis of the causative agent of Chagas disease: Trypanosoma cruzi
International Journal for Parasitology
A Parodi-TaliceC Robello
Abstract
Trypanosoma cruzi, the causative agent of Chagas disease, has evolved particular mechanisms of gene regulation. Gene expression is regulated firstly at post-transcriptional level. This feature makes proteomic methods a promising tool for studying adaptative changes in these parasites. In this work we generated a reproducible method for protein analysis by two-dimensional electrophoresis coupled to mass spectrometry, and a protein map for T. cruzi. Western-blot analysis supported the identity of some of the proteins. This work points to proteomic approach as a powerful tool to study differential expression, stress response or drug resistance in T. cruzi.
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