Proteome analysis of tissues by mass spectrometry

Mass Spectrometry Reviews
Irena DapicGarry L Corthals

Abstract

Tissues and biofluids are important sources of information used for the detection of diseases and decisions on patient therapies. There are several accepted methods for preservation of tissues, among which the most popular are fresh-frozen and formalin-fixed paraffin embedded methods. Depending on the preservation method and the amount of sample available, various specific protocols are available for tissue processing for subsequent proteomic analysis. Protocols are tailored to answer various biological questions, and as such vary in lysis and digestion conditions, as well as duration. The existence of diverse tissue-sample protocols has led to confusion in how to choose the best protocol for a given tissue and made it difficult to compare results across sample types. Here, we summarize procedures used for tissue processing for subsequent bottom-up proteomic analysis. Furthermore, we compare protocols for their variations in the composition of lysis buffers, digestion procedures, and purification steps. For example, reports have shown that lysis buffer composition plays an important role in the profile of extracted proteins: the most common are tris(hydroxymethyl)aminomethane, radioimmunoprecipitation assay, and ammonium bicarb...Continue Reading

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Citations

Jun 25, 2020·Proteomics·Ferdinand von Eggeling, Franziska Hoffmann
Jul 17, 2020·Frontiers in Aging Neuroscience·Mor Savyon, Simone Engelender
Jan 26, 2021·Analytical Methods : Advancing Methods and Applications·Chang LiuLijiao Zhao
Nov 5, 2020·International Journal of Molecular Sciences·Andrew ShapanisPaul Skipp
May 28, 2021·The Analyst·Yiheng MaoRuijun Tian

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