Proteome analysis reveals global response to deletion of mrflbA in Monascus ruber

The Journal of Microbiology
Qingqing YanYanchun Shao

Abstract

Monascus spp. are commonly used for a wide variety of applications in the food and pharmaceutical industries. In previous studies, the knock-out of mrflbA (a putative regulator of the G protein α subunit) in M. ruber led to autolysis of the mycelia, decreased pigmentation and lowered mycotoxin production. Therefore, we aimed to obtain a comprehensive overview of the underlying mechanism of mrflbA deletion at the proteome level. A two-dimensional gel electrophoresis analysis of mycelial proteins indicated that the abundance of 178 proteins was altered in the ΔmrflbA strain, 33 of which were identified with high confidence. The identified proteins are involved in a range of activities, including carbohydrate and amino acid metabolism, hyphal development and the oxidative stress response, protein modification, and the regulation of cell signaling. Consistent with these findings, the activity of antioxidative enzymes and chitinase was elevated in the supernatant of the ΔmrflbA strain. Furthermore, deletion of mrflbA resulted in the transcriptional reduction of secondary metabolites (pigment and mycotoxin). In short, the mutant phenotypes induced by the deletion of mrflbA were consistent with changes in the expression levels of asso...Continue Reading

References

Oct 11, 2007·Journal of Agricultural and Food Chemistry·Wun-Yuan LinTzu-Ming Pan
Dec 22, 2007·Journal of Agricultural and Food Chemistry·Wun-Yuan LinTzu-Ming Pan
Sep 3, 2008·Molecular Microbiology·Chaoyang XueJoseph Heitman
Nov 1, 2008·Applied Biochemistry and Biotechnology·Tamás EmriIstván Pócsi
May 29, 2012·Fungal Genetics and Biology : FG & B·Ae Ran ParkYin-Won Lee
Nov 28, 2012·Journal of Industrial Microbiology & Biotechnology·Petra Patakova
Jun 20, 2013·Nature Reviews. Molecular Cell Biology·Jose Javier Bravo-CorderoJohn Condeelis
Mar 19, 2014·Applied Microbiology and Biotechnology·Yanchun ShaoFusheng Chen
Dec 9, 2014·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Yan-Ping LiQing-Hua He
Mar 13, 2015·Frontiers in Microbiology·Frédéric LamothWilliam J Steinbach
Sep 30, 2017·Chemical Science·Wanping ChenFusheng Chen

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