Proteome annotations and identifications of the human pulmonary fibroblast

Journal of Proteome Research
Johan MalmströmGyörgy Marko-Varga

Abstract

We hereby report on a three year project initiative undertaken by our research team encompassing large-scale protein expression profiling and annotations of human primary lung fibroblast cells. An overview is given of proteomic studies of the fibroblast target cell involved in several diseases such as asthma, idiopatic pulmonary disease, and COPD. It has been the objective within our research team to map and identify the protein expressions occurring in both activated-, as well as resting cell states. The JGGL database www.2DDB.org has been built around these data, allowing advanced hypothesis building using the interactive query bioinformatic tools developed. Gene ontology has been applied to these annotations, classifying and correlating protein expressions to function. The localization as well as the biological processes involved for the annotations are being presented including an annotation-, and sequence-identification strategy, resulting in close to 2000 protein identities. Both gel based, high resolution 2D-gels, and liquid-phase separation (three-dimensional HPLC), as well as the combination of gel- and LC-based approaches (1D-gels and nano-capillary LC, reversed-phase) were utilized. Protein sequencing and structure i...Continue Reading

References

Aug 1, 1992·American Journal of Respiratory Cell and Molecular Biology·D J RombergerS I Rennard
May 1, 1969·Journal of Ultrastructure Research·A Monneron, W Bernhard
Mar 1, 1995·Chest·C R Roberts
Feb 1, 1993·The American Review of Respiratory Disease·N CarrollA James
Jun 13, 1996·Nature·D SabbertW Junge
Aug 1, 1997·American Journal of Human Genetics·T A Cooper, W Mattox
Oct 6, 1998·Annual Review of Biochemistry·J L Workman, R E Kingston
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·N T LiberatiX F Wang
Mar 11, 2000·Molecular Cell·C DongP J Goldschmidt-Clermont
Aug 1, 2000·Trends in Biochemical Sciences·M A Schwartz, S J Shattil
Dec 9, 2000·American Journal of Respiratory and Critical Care Medicine·C OrdoñezJ V Fahy
Jan 9, 2001·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·N BeckmannJ R Fozard
Feb 22, 2001·Science·J C VenterX Zhu
Mar 15, 2001·Journal of Pharmaceutical and Biomedical Analysis·G Westergren-ThorssonG Marko-Varga
Jun 8, 2001·Annual Review of Biochemistry·M MannA Pandey
Jun 27, 2001·Electrophoresis·J MalmströmG Marko-Varga
Aug 3, 2001·The EMBO Journal·S Weg-RemersH König
Nov 20, 2001·Chemical Reviews·R Aebersold, D R Goodlett
Dec 6, 2001·American Journal of Respiratory and Critical Care Medicine·P K Jeffery
Dec 26, 2001·Nature Genetics·Barmak Modrek, Christopher Lee
Jan 16, 2002·Current Biology : CB·Jens S AndersenAngus I Lamond
Jan 31, 2002·Annual Review of Medicine·Lee Maddox, David A Schwartz
May 4, 2002·Nature Reviews. Molecular Cell Biology·James J TomasekRobert A Brown
Aug 3, 2002·European Journal of Biochemistry·Ellen TufvessonGunilla Westergren-Thorsson
Aug 15, 2002·Genome Research·Juri RappsilberMatthias Mann
Aug 28, 2002·The Journal of Cell Biology·Janet M CronshawMichael J Matunis
Sep 7, 2002·Journal of Molecular Biology·Richard BonneauDavid Baker
Oct 16, 2002·The International Journal of Biochemistry & Cell Biology·Annie Pardo, Moisés Selman

❮ Previous
Next ❯

Citations

Feb 3, 2011·Lung·Lina María Salazar, Ana Milena Herrera
Sep 5, 2003·Journal of the American Society for Mass Spectrometry·Wanda M BodnarM Arthur Moseley
Jun 8, 2007·Expert Review of Proteomics·Praveen GovenderSeamas C Donnelly
Oct 9, 2009·COPD·Benjamin L NicholasRatko Djukanovic
Oct 1, 2007·Expert Review of Respiratory Medicine·Stewart J Levine
Jun 14, 2005·Molecular & Cellular Proteomics : MCP·Jiang WuMary Jane Thomassen
Nov 30, 2006·Proteomics·Barbara MagiPaola Rottoli
Apr 27, 2005·Proteomics·Harvey B PollardDavid M Jacobowitz
Oct 22, 2008·Expert Review of Proteomics·Begona CasadoMartin Kussmann
Dec 18, 2010·Expert Review of Clinical Immunology·Begoña CasadoPaolo Iadarola
Jul 21, 2017·Clinical Oral Investigations·Ponlatham ChaiyaritJan Potempa
Jan 22, 2021·European Respiratory Review : an Official Journal of the European Respiratory Society·Wytse B van den BoschHarm A W M Tiddens
Jul 20, 2007·Journal of Proteome Research·György Marko-VargaHarubumi Kato
Apr 27, 2010·Chemical Reviews·Akos Végvári, György Marko-Varga
Dec 15, 2004·Journal of Proteome Research·Robertson CraigRonald C Beavis
Dec 13, 2005·Journal of Proteome Research·Stephen J HattanPeter Juhasz
Aug 9, 2005·Journal of Proteome Research·György Marko-VargaThomas E Fehniger

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.