Proteome-based comparative analyses of growth stages reveal new cell cycle-dependent functions in the predatory bacterium Bdellovibrio bacteriovorus

Applied and Environmental Microbiology
Mally Dori-BachashÉdouard Jurkevitch

Abstract

Bdellovibrio and like organisms are obligate predators of bacteria that are ubiquitously found in the environment. Most exhibit a peculiar dimorphic life cycle during which free-swimming attack-phase (AP) cells search for and invade bacterial prey cells. The invader develops in the prey as a filamentous polynucleoid-containing cell that finally splits into progeny cells. Therapeutic and biocontrol applications of Bdellovibrio in human and animal health and plant health, respectively, have been proposed, but more knowledge of this peculiar cell cycle is needed to develop such applications. A proteomic approach was applied to study cell cycle-dependent expression of the Bdellovibrio bacteriovorus proteome in synchronous cultures of a facultative host-independent (HI) strain able to grow in the absence of prey. Results from two-dimensional gel electrophoresis, mass spectrometry, and temporal expression of selected genes in predicted operons were analyzed. In total, about 21% of the in silico predicted proteome was covered. One hundred ninety-six proteins were identified, including 63 hitherto unknown proteins and 140 life stage-dependent spots. Of those, 47 were differentially expressed, including chemotaxis, attachment, growth- a...Continue Reading

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Citations

Jan 21, 2009·Functional & Integrative Genomics·Mally Dori-BachashShmuel Pietrokovski
Nov 30, 2012·The ISME Journal·Zohar PasternakÉdouard Jurkevitch
Jul 10, 2012·Applied and Environmental Microbiology·Hui YangDafang Huang
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Oct 4, 2013·The ISME Journal·Zohar PasternakÉdouard Jurkevitch
Oct 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Or RotemÉdouard Jurkevitch
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Sep 2, 2015·Journal of Bacteriology·Or RotemÉdouard Jurkevitch
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May 1, 2020·Frontiers in Microbiology·Eleni BratanisEwa Bukowska-Faniband
Jun 15, 2017·Médecine sciences : M/S·Édouard Jurkevitch, Stéphan Jacquet

Related Concepts

Bacterial Proteins
Bdellovibrio
Mass Spectrometry
2D Polyacrylamide Gel Electrophoresis
Proteome
MRNA Differential Display
Bdellovibrio
Cell Cycle
Cell Wall
Chemotaxis

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