Proteome changes during yeast-like and pseudohyphal growth in the biofilm-forming yeast Pichia fermentans

Amino Acids
Biancaelena MasertiQuirico Migheli

Abstract

The Pichia fermentans strain DISAABA 726 is a biofilm-forming yeast that has been proposed as biocontrol agent to control brown rot on apple. How ever, when inoculated on peach, strain 726 shows yeast-like to pseudohyphal transition coupled to a pathogenic behaviour. To identify the proteins potentially involved in such transition process, a comparative proteome analysis of P. fermentans 726 developed on peach (filamentous growth) vs apple (yeast-like growth) was carried out using two-dimensional gel electrophoresis coupled with mass spectrometry analysis. The proteome comparison was also performed between the two different cell morphologies induced in a liquid medium amended with urea (yeast-like cells) or methionine (filamentous cells) to exclude fruit tissue impact on the transition. Seventy-three protein spots showed significant variations in abundance (±twofold, p < 0.01, confidence intervals 99 %) between pseudohyphal vs yeast-like morphology produced on fruits. Among them, 30 proteins changed their levels when the two morphologies were developed in liquid medium. The identified proteins belong to several pathways and functions, such as glycolysis, amino acid synthesis, chaperones, and signalling transduction. The possibl...Continue Reading

References

Jun 1, 1996·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·H Han-YakuT Nishikawa
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·B N Lee, E A Elion
Jan 19, 2000·Molecular Biology of the Cell·M C LorenzJ Heitman
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·H FuS C Masters
Jan 12, 2001·FEMS Microbiology Reviews·J M Gancedo
Oct 13, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M J van HemertG P van Heusden
Jul 4, 2002·Molecular & Cellular Proteomics : MCP·Guoan ChenDavid G Beer
Mar 1, 2005·Bioinformatics·Alexei A SharovMinoru S H Ko
Feb 19, 2008·Applied and Environmental Microbiology·Lucie A HazelwoodJ Richard Dickinson
May 16, 2008·Folia Microbiologica·J R Dickinson
Jul 22, 2008·Chemistry Central Journal·Peter R JungblutHartmut Schlüter
Feb 17, 2009·Journal of Proteome Research·Martin DragositsDiethard Mattanovich
Sep 11, 2009·Chemistry Central Journal·Hartmut SchlüterPeter R Jungblut
Oct 12, 2010·Acta Biochimica Et Biophysica Sinica·Xiaoyan LiuJiangye Chen
Dec 8, 2010·Journal of Proteome Research·Lucia MonteolivaConcha Gil
Jul 8, 2011·Postȩpy higieny i medycyny doświadczalnej·Adam Tylicki, Magdalena Siemieniuk
Jul 4, 2012·Fungal Biology·Maria Lina SannaIlaria Mannazzu
Jul 17, 2012·Molecular Genetics and Genomics : MGG·Agustina Olivera-Couto, Pablo S Aguilar
Dec 4, 2012·Nucleic Acids Research·Juan Antonio VizcaínoHenning Hermjakob
Apr 15, 2014·Nature Biotechnology·Juan A VizcaínoHenning Hermjakob

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Citations

Dec 8, 2015·Environmental Science and Pollution Research International·Guangkun LiJia Liu
Mar 4, 2017·Journal of Cellular Physiology·Federica LeoneRenata Del Carratore
Oct 3, 2019·World Journal of Microbiology & Biotechnology·Florian M FreimoserQuirico Migheli

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